Cofilin activity during insulin-like growth factor I-stimulated neuroblastoma cell motility
dc.contributor.author | Meyer, Gary | en_US |
dc.contributor.author | Kim, Bhumsoo | en_US |
dc.contributor.author | Golen, C. | en_US |
dc.contributor.author | Feldman, Eva L. | en_US |
dc.date.accessioned | 2006-09-08T20:49:48Z | |
dc.date.available | 2006-09-08T20:49:48Z | |
dc.date.issued | 2005-02 | en_US |
dc.identifier.citation | Meyer, G.; Kim, B.; Golen, C.; Feldman, E. L.; (2005). "Cofilin activity during insulin-like growth factor I-stimulated neuroblastoma cell motility." CMLS Cellular and Molecular Life Sciences 62(4): 461-470. <http://hdl.handle.net/2027.42/42884> | en_US |
dc.identifier.issn | 1420-682X | en_US |
dc.identifier.issn | 1420-9071 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/42884 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=15719172&dopt=citation | en_US |
dc.description.abstract | Insulin-like growth factor I (IGF-I) is a potent stimulator of neuroblastoma cell motility. Cell motility requires lamellipodium extension at the leading edge of the cell through organized actin polymerization, and IGF-I stimulates lamellipodial elaboration in human neuroblastoma cells. Rac is a Rho GTPase that stimulates lamellipodial formation via the regulation of actin polymerization. In this study, we show that IGF-I-stimulated phosphatidylinositol 3-kinase (PI-3K) activity promotes rac activation and subsequent activation of the down- stream effectors LIM kinase and cofilin. Overexpression of wild-type LIM kinase and wild-type Xenopus ADF/cofilin (XAC) suppresses IGF-I-stimulated motility in SH-SY5Y cells, while expression of dominant negative LIM kinase and constitutively active XAC increases SH-SY5Y motility in the absence of IGF-I stimulation. These results suggest that regulation by cofilin of actin depolymerization is important in the process of neuroblastoma cell motility, and IGF-I regulates cofilin activity in part through PI-3K, rac, and LIM kinase. | en_US |
dc.format.extent | 369351 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Birkhäuser-Verlag; Birkhäuser Verlag, Basel ; Springer Science+Business Media | en_US |
dc.subject.other | Life Sciences | en_US |
dc.subject.other | Biomedicine General | en_US |
dc.subject.other | Life Sciences, General | en_US |
dc.subject.other | Biochemistry, General | en_US |
dc.subject.other | Cell Biology | en_US |
dc.subject.other | LIMK | en_US |
dc.subject.other | Rac | en_US |
dc.subject.other | Cytoskeleton | en_US |
dc.subject.other | Tumor | en_US |
dc.subject.other | PAK | en_US |
dc.title | Cofilin activity during insulin-like growth factor I-stimulated neuroblastoma cell motility | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Science (General) | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Neurology, University of Michigan, 4414 Kresge III, 200 Zina Pitcher Place, Ann Arbor, Michigan, 48109, USA | en_US |
dc.contributor.affiliationum | Department of Neurology, University of Michigan, 4414 Kresge III, 200 Zina Pitcher Place, Ann Arbor, Michigan, 48109, USA | en_US |
dc.contributor.affiliationum | Department of Neurology, University of Michigan, 4414 Kresge III, 200 Zina Pitcher Place, Ann Arbor, Michigan, 48109, USA | en_US |
dc.contributor.affiliationum | Department of Neurology, University of Michigan, 4414 Kresge III, 200 Zina Pitcher Place, Ann Arbor, Michigan, 48109, USA | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 15719172 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/42884/1/18_2004_Article_4456.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/s00018-004-4456-6 | en_US |
dc.identifier.source | CMLS Cellular and Molecular Life Sciences | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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