View Item 

Show simple item record

High-Resolution Detection of Five Frequencies in a Single 3D Spectrum: HNHCACO - a Bidirectional Coherence Transfer Experiment
dc.contributor.authorPang, Yuxien_US
dc.contributor.authorZeng, Leien_US
dc.contributor.authorKurochkin, Alexander V.en_US
dc.contributor.authorZuiderweg, Erik R. P.en_US
dc.date.accessioned2006-09-08T21:00:11Z
dc.date.available2006-09-08T21:00:11Z
dc.date.issued1998-02en_US
dc.identifier.citationPang, Yuxi; Zeng, Lei; Kurochkin, Alexander V.; Zuiderweg, Erik R.P.; (1998). "High-Resolution Detection of Five Frequencies in a Single 3D Spectrum: HNHCACO - a Bidirectional Coherence Transfer Experiment." Journal of Biomolecular NMR 11(2): 185-190. <http://hdl.handle.net/2027.42/43041>en_US
dc.identifier.issn0925-2738en_US
dc.identifier.issn1573-5001en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/43041
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=9679293&dopt=citationen_US
dc.description.abstractA new triple-resonance pulse sequence, 3D HNHCACO, is introduced and discussed, which identifies sequential correlations of the backbone nuclei (Hα(i-1), Cα(i-1), C′(i-1), NH(i)) of doubly labeled proteins in H2O. The three-dimensional (3D) method utilizes a recording of 15N and 13C resonances in a single indirect time domain, the 13C′ resonance in another indirect time domain, and detects both NH and Hα protons. A bidirectional coherence transfer (NH(i) ↮ N(i) ↮ C′(i-1) ↮ Cα(i-1) ↮ Hα(i-1)) is effectuated, resulting in a single high-resolution 3D spectrum that contains the frequencies of all five backbone nuclei. The experiment was applied to the 12.3 kDa ribonuclease from Bacillus intermedius (Binase).en_US
dc.format.extent306005 bytes
dc.format.extent3115 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherKluwer Academic Publishers; Springer Science+Business Mediaen_US
dc.subject.otherChemistryen_US
dc.subject.otherPolymer Sciencesen_US
dc.subject.otherAnimal Anatomy / Morphology / Histologyen_US
dc.subject.otherBidirectional Coherence Transferen_US
dc.subject.otherBinaseen_US
dc.subject.otherGradient Coherence Selectionen_US
dc.subject.otherHNHCACOen_US
dc.subject.otherSimultaneous 15N and 13Cα Acquisitionen_US
dc.titleHigh-Resolution Detection of Five Frequencies in a Single 3D Spectrum: HNHCACO - a Bidirectional Coherence Transfer Experimenten_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbsecondlevelNatural Resources and Environmenten_US
dc.subject.hlbsecondlevelEcology and Evolutionary Biologyen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.subject.hlbtoplevelScienceen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumBiophysics Research Division, University of Michigan, 930 N. University Avenue, Ann Arbor, MI, 48109-1055, U.S.Aen_US
dc.contributor.affiliationumBiophysics Research Division, University of Michigan, 930 N. University Avenue, Ann Arbor, MI, 48109-1055, U.S.Aen_US
dc.contributor.affiliationumBiophysics Research Division, University of Michigan, 930 N. University Avenue, Ann Arbor, MI, 48109-1055, U.S.Aen_US
dc.contributor.affiliationumBiophysics Research Division, University of Michigan, 930 N. University Avenue, Ann Arbor, MI, 48109-1055, U.S.A; Department of Biological Chemistry, University of Michigan, 930 N. University Avenue, Ann Arbor, MI, 48109-1055, U.S.A.; Department of Chemistry, University of Michigan, 930 N. University Avenue, Ann Arbor, MI, 48109-1055, U.S.Aen_US
dc.contributor.affiliationumcampusAnn Arboren_US
dc.identifier.pmid9679293en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/43041/1/10858_2004_Article_152277.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1023/A:1008229723544en_US
dc.identifier.sourceJournal of Biomolecular NMRen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


Files in this item

Name:
10858_2004_Article_152277.pdf
Size:
298.8KB
Format:
PDF
View/Open

Show simple item record

Remediation of Harmful Language

The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.

Accessibility

If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.