High-Resolution Detection of Five Frequencies in a Single 3D Spectrum: HNHCACO - a Bidirectional Coherence Transfer Experiment
dc.contributor.author | Pang, Yuxi | en_US |
dc.contributor.author | Zeng, Lei | en_US |
dc.contributor.author | Kurochkin, Alexander V. | en_US |
dc.contributor.author | Zuiderweg, Erik R. P. | en_US |
dc.date.accessioned | 2006-09-08T21:00:11Z | |
dc.date.available | 2006-09-08T21:00:11Z | |
dc.date.issued | 1998-02 | en_US |
dc.identifier.citation | Pang, Yuxi; Zeng, Lei; Kurochkin, Alexander V.; Zuiderweg, Erik R.P.; (1998). "High-Resolution Detection of Five Frequencies in a Single 3D Spectrum: HNHCACO - a Bidirectional Coherence Transfer Experiment." Journal of Biomolecular NMR 11(2): 185-190. <http://hdl.handle.net/2027.42/43041> | en_US |
dc.identifier.issn | 0925-2738 | en_US |
dc.identifier.issn | 1573-5001 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/43041 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=9679293&dopt=citation | en_US |
dc.description.abstract | A new triple-resonance pulse sequence, 3D HNHCACO, is introduced and discussed, which identifies sequential correlations of the backbone nuclei (Hα(i-1), Cα(i-1), C′(i-1), NH(i)) of doubly labeled proteins in H2O. The three-dimensional (3D) method utilizes a recording of 15N and 13C resonances in a single indirect time domain, the 13C′ resonance in another indirect time domain, and detects both NH and Hα protons. A bidirectional coherence transfer (NH(i) ↮ N(i) ↮ C′(i-1) ↮ Cα(i-1) ↮ Hα(i-1)) is effectuated, resulting in a single high-resolution 3D spectrum that contains the frequencies of all five backbone nuclei. The experiment was applied to the 12.3 kDa ribonuclease from Bacillus intermedius (Binase). | en_US |
dc.format.extent | 306005 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Kluwer Academic Publishers; Springer Science+Business Media | en_US |
dc.subject.other | Chemistry | en_US |
dc.subject.other | Polymer Sciences | en_US |
dc.subject.other | Animal Anatomy / Morphology / Histology | en_US |
dc.subject.other | Bidirectional Coherence Transfer | en_US |
dc.subject.other | Binase | en_US |
dc.subject.other | Gradient Coherence Selection | en_US |
dc.subject.other | HNHCACO | en_US |
dc.subject.other | Simultaneous 15N and 13Cα Acquisition | en_US |
dc.title | High-Resolution Detection of Five Frequencies in a Single 3D Spectrum: HNHCACO - a Bidirectional Coherence Transfer Experiment | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Biophysics Research Division, University of Michigan, 930 N. University Avenue, Ann Arbor, MI, 48109-1055, U.S.A | en_US |
dc.contributor.affiliationum | Biophysics Research Division, University of Michigan, 930 N. University Avenue, Ann Arbor, MI, 48109-1055, U.S.A | en_US |
dc.contributor.affiliationum | Biophysics Research Division, University of Michigan, 930 N. University Avenue, Ann Arbor, MI, 48109-1055, U.S.A | en_US |
dc.contributor.affiliationum | Biophysics Research Division, University of Michigan, 930 N. University Avenue, Ann Arbor, MI, 48109-1055, U.S.A; Department of Biological Chemistry, University of Michigan, 930 N. University Avenue, Ann Arbor, MI, 48109-1055, U.S.A.; Department of Chemistry, University of Michigan, 930 N. University Avenue, Ann Arbor, MI, 48109-1055, U.S.A | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 9679293 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/43041/1/10858_2004_Article_152277.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1023/A:1008229723544 | en_US |
dc.identifier.source | Journal of Biomolecular NMR | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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