A new 3D HN(CA)HA experiment for obtaining fingerprint H N -H α cross peaks in 15 N- and 13 C-labeled proteins
dc.contributor.author | Clubb, Robert T. | en_US |
dc.contributor.author | Thanabal, Venkataraman | en_US |
dc.contributor.author | Wagner, Gerhard | en_US |
dc.date.accessioned | 2006-09-08T21:00:20Z | |
dc.date.available | 2006-09-08T21:00:20Z | |
dc.date.issued | 1992-03 | en_US |
dc.identifier.citation | Clubb, Robert T.; Thanabal, V.; Wagner, Gerhard; (1992). "A new 3D HN(CA)HA experiment for obtaining fingerprint H N -H α cross peaks in 15 N- and 13 C-labeled proteins." Journal of Biomolecular NMR 2(2): 203-210. <http://hdl.handle.net/2027.42/43043> | en_US |
dc.identifier.issn | 0925-2738 | en_US |
dc.identifier.issn | 1573-5001 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/43043 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=1422153&dopt=citation | en_US |
dc.description.abstract | A new 3D 1 H− 15 N− 13 C triple resonance experiment is presented that provides in-phase absorptive cross peaks between amide protons and α-protons of the same and the sequentially preceding residue. The experiment yields similar connectivities as those described previously by Montelione and Wagner (1990a) [ J. Magn. Reson. , 87 , 183–188] and Kay et al. (1991) [ J. Magn. Reson. , 91 , 84–92]. However, the pulse sequence was designed to minimize the time that transverse coherence of the 13 C α nucleus is present, since this nucleus has the shortest transverse relaxation time of all the nuclei involved in these experiments. This is achieved by using a coherence transfer pathway from 1 H N to 15 N, 13 C α , 1 H α and back to the 1 H N . In the sequence described, transverse 13 C α coherence is present only for a length of ca. I 1 J(C α -H α ). This reduces loss of signal due to transverse relaxation. We tested the technique on uniformly 15 N- and 13 C-enriched T4 lysozyme. | en_US |
dc.format.extent | 436164 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Kluwer Academic Publishers; ESCOM Science Publishers B.V. ; Springer Science+Business Media | en_US |
dc.subject.other | Chemistry | en_US |
dc.subject.other | Polymer Sciences | en_US |
dc.subject.other | Biochemistry, General | en_US |
dc.subject.other | Biophysics/Biomedical Physics | en_US |
dc.subject.other | 3D NMR | en_US |
dc.subject.other | J Connectivity | en_US |
dc.subject.other | Resonance Assignment | en_US |
dc.subject.other | Isotope Labeling | en_US |
dc.subject.other | Proteins | en_US |
dc.subject.other | T4 Lysozyme | en_US |
dc.title | A new 3D HN(CA)HA experiment for obtaining fingerprint H N -H α cross peaks in 15 N- and 13 C-labeled proteins | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, 02115, Boston, MA, USA; Department of Biological Chemistry, University of Michigan, 1301 Catherine Street, 48109, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationother | Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, 02115, Boston, MA, USA | en_US |
dc.contributor.affiliationother | Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, 02115, Boston, MA, USA | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 1422153 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/43043/1/10858_2005_Article_BF01875531.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/BF01875531 | en_US |
dc.identifier.source | Journal of Biomolecular NMR | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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