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Sensitivity improvement in 2D and 3D HCCH spectroscopy using heteronuclear cross-polarization
dc.contributor.authorMajumdar, Ananyaen_US
dc.contributor.authorWang, Hongen_US
dc.contributor.authorMorshauser, Robert C.en_US
dc.contributor.authorZuiderweg, Erik R. P.en_US
dc.date.accessioned2006-09-08T21:00:36Z
dc.date.available2006-09-08T21:00:36Z
dc.date.issued1993-07en_US
dc.identifier.citationMajumdar, Ananya; Wang, Hong; Morshauser, Robert C.; Zuiderweg, Erik R. P.; (1993). "Sensitivity improvement in 2D and 3D HCCH spectroscopy using heteronuclear cross-polarization." Journal of Biomolecular NMR 3(4): 387-397. <http://hdl.handle.net/2027.42/43047>en_US
dc.identifier.issn0925-2738en_US
dc.identifier.issn1573-5001en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/43047
dc.description.abstractA new method, which employs a sequence of heteronuclear-homonuclear-heteronuclear Hartmann-Hahn (HEHOHEHAHA) cross-polarization steps for obtaining through-bond H-C-C-H correlations in larger proteins (M r > 15 kDa), is presented. The method has significantly higher sensitivity compared to INEPTHOHAHA-INEPT-based techniques. An additional feature of this experiment is that well-phaseable spectra may be obtained with a minimal (4-step) phase cycle and, consequently, experimental time can be utilized towards obtaining high resolution in indirect dimensions. Results from 2D and 3D HEHOHEHAHA experiments on T4-lysozyme are presented.en_US
dc.format.extent654936 bytes
dc.format.extent3115 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherKluwer Academic Publishers; ESCOM Science Publishers B.V. ; Springer Science+Business Mediaen_US
dc.subject.otherChemistryen_US
dc.subject.otherPolymer Sciencesen_US
dc.subject.otherAnimal Anatomy / Morphology / Histologyen_US
dc.subject.otherHeteronuclear Cross Polarizationen_US
dc.subject.otherHCCHen_US
dc.subject.otherHOHAHAen_US
dc.subject.otherTOCSYen_US
dc.subject.otherT4-lysozymeen_US
dc.subject.otherLabeled Proteinsen_US
dc.subject.otherComposite Pulse Sequencesen_US
dc.titleSensitivity improvement in 2D and 3D HCCH spectroscopy using heteronuclear cross-polarizationen_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelNatural Resources and Environmenten_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbsecondlevelEcology and Evolutionary Biologyen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.subject.hlbtoplevelScienceen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumBiophysics Research Division, The University of Michigan, 930 North University, 48109-1055, Ann Arbor, MI, U.S.A.en_US
dc.contributor.affiliationumBiophysics Research Division, The University of Michigan, 930 North University, 48109-1055, Ann Arbor, MI, U.S.A.en_US
dc.contributor.affiliationumBiophysics Research Division, The University of Michigan, 930 North University, 48109-1055, Ann Arbor, MI, U.S.A.en_US
dc.contributor.affiliationumBiophysics Research Division, The University of Michigan, 930 North University, 48109-1055, Ann Arbor, MI, U.S.A.en_US
dc.contributor.affiliationumcampusAnn Arboren_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/43047/1/10858_2004_Article_BF00176006.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1007/BF00176006en_US
dc.identifier.sourceJournal of Biomolecular NMRen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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