A technique for the determination of protein concentration by neutron activation analysis of silver binding
dc.contributor.author | Frasch, Wayne D. | en_US |
dc.contributor.author | Larsen, J. | en_US |
dc.contributor.author | Bowlby, Neil R. | en_US |
dc.contributor.author | Apel, Ingrid J. | en_US |
dc.contributor.author | Jones, John D. | en_US |
dc.date.accessioned | 2006-09-08T21:04:42Z | |
dc.date.available | 2006-09-08T21:04:42Z | |
dc.date.issued | 1987-05 | en_US |
dc.identifier.citation | Frasch, W. D.; Larsen, J.; Bowlby, N.; Apel, I.; Jones, J. D.; (1987). "A technique for the determination of protein concentration by neutron activation analysis of silver binding." Journal of Radioanalytical and Nuclear Chemistry Articles 112(1): 89-94. <http://hdl.handle.net/2027.42/43109> | en_US |
dc.identifier.issn | 0236-5731 | en_US |
dc.identifier.issn | 1588-2780 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/43109 | |
dc.description.abstract | A method for the quantitative determination of small amounts of protein samples was developed employing neutron activation analysis. Current methods of protein concentration determination are severely limited as a result of differences in the specific characteristics of each protein. Silver binding has been used as a sensitive colorimetric method to indicate the presence of protein. However, silver-protein complexes can have a variety of absorbance spectra unique to each protein, which complicate the analysis. Various amounts of specific proteins were equilibrated in an excess of silver nitrate prior to the reduction of the silver by the addition of NaBH 4 , HCHO, and NaOH. The protein-silver complex was rapidly separated from the unbound silver by centrifugation chromatography and the amount of bound silver was determined by INAA. The amount of silver was proportional to the amount of protein present in each sample. When the silver was not reduced prior to removal of the unbound silver by chromatography, only negligible amounts of silver remained bound to the protein. The stoichiometry of bound silver to protein on a molar basis showed relatively small differences for the proteins that were examined. This ratio was found to depend on the conditions of the binding and reduction of the silver. The results suggest that the binding of silver is not specific to any charged or polar groups on these proteins and may, therefore, provide a means of determination of the concentration of protein that has general application for all proteins. | en_US |
dc.format.extent | 322978 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Kluwer Academic Publishers; Akadémiai Kiadó ; Springer Science+Business Media | en_US |
dc.subject.other | Chemistry | en_US |
dc.subject.other | Inorganic Chemistry | en_US |
dc.subject.other | Physical Chemistry | en_US |
dc.subject.other | Diagnostic Radiology | en_US |
dc.subject.other | Nuclear Physics, Heavy Ions, Hadrons | en_US |
dc.subject.other | Nuclear Chemistry | en_US |
dc.title | A technique for the determination of protein concentration by neutron activation analysis of silver binding | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Chemical Engineering | en_US |
dc.subject.hlbsecondlevel | Chemistry | en_US |
dc.subject.hlbsecondlevel | Materials Science and Engineering | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | The Division of Biological Sciences, The University of Michigan, 48109, Ann Arbor, MI, (USA) | en_US |
dc.contributor.affiliationum | The Division of Biological Sciences, The University of Michigan, 48109, Ann Arbor, MI, (USA) | en_US |
dc.contributor.affiliationum | The Division of Biological Sciences, The University of Michigan, 48109, Ann Arbor, MI, (USA) | en_US |
dc.contributor.affiliationum | The Division of Biological Sciences, The University of Michigan, 48109, Ann Arbor, MI, (USA) | en_US |
dc.contributor.affiliationum | The Phoenix Memorial Laboratory, The University of Michigan, 48109, Ann Arbor, MI, (USA) | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/43109/1/10967_2005_Article_BF02037279.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/BF02037279 | en_US |
dc.identifier.source | Journal of Radioanalytical and Nuclear Chemistry Articles | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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