Methylation of somatic and sperm DNA in the homosporous fern Ceratopteris richardii
dc.contributor.author | McGrath, J. Mitchell | en_US |
dc.contributor.author | Pichersky, Eran | en_US |
dc.date.accessioned | 2006-09-08T21:27:03Z | |
dc.date.available | 2006-09-08T21:27:03Z | |
dc.date.issued | 1997-12 | en_US |
dc.identifier.citation | McGrath, J. Mitchell; Pichersky, Eran; (1997). "Methylation of somatic and sperm DNA in the homosporous fern Ceratopteris richardii." Plant Molecular Biology 35(6): 1023-1027. <http://hdl.handle.net/2027.42/43445> | en_US |
dc.identifier.issn | 0167-4412 | en_US |
dc.identifier.issn | 1573-5028 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/43445 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=9426624&dopt=citation | en_US |
dc.description.abstract | Plants, in general, have a high proportion of their CpG and CpNpG nucleotide motifs modified with 5-methylcytosine (5mC). Developmental changes in the proportion of 5mC are evident in mammals, particularly during gametogenesis and embryogenesis, but little information is available from flowering plants due to the intimate association of gametes with sporophytic tissues. In ferns, sperm are uninucleate and free-swimming and thus are easily isolated. We have examined 5mC in DNA isolated from fern sperm and other tissues with methylation-sensitive and -insensitive restriction enzyme isoschizomers, Southern blots probed with chloroplast and nuclear ribosomal RNA genes and end-labeled restriction fragments. We conclude that fern sperm DNA is methylated to a similar or greater degree than DNA isolated from either sporophytes or gametophytes. | en_US |
dc.format.extent | 266725 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Kluwer Academic Publishers; Springer Science+Business Media | en_US |
dc.subject.other | Life Sciences | en_US |
dc.subject.other | Biochemistry, General | en_US |
dc.subject.other | Plant Sciences | en_US |
dc.subject.other | Plant Pathology | en_US |
dc.subject.other | Somatic DNA | en_US |
dc.subject.other | Homosporous Fern | en_US |
dc.subject.other | Sperm DNA | en_US |
dc.title | Methylation of somatic and sperm DNA in the homosporous fern Ceratopteris richardii | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbsecondlevel | Genetics | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biology, University of Michigan, Ann Arbor, MI, 48109-1048, USA | en_US |
dc.contributor.affiliationother | Department of Crop and Soil Sciences, Michigan State University, East Lansing, MI, 48824–1325, USA | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 9426624 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/43445/1/11103_2004_Article_148087.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1023/A:1005962520544 | en_US |
dc.identifier.source | Plant Molecular Biology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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