Mn 2+ reduces Y z + in manganese-depleted Photosystem II preparations
dc.contributor.author | Hoganson, Curtis W. | en_US |
dc.contributor.author | Ghanotakis, Demetrios F. | en_US |
dc.contributor.author | Babcock, Gerald T. | en_US |
dc.contributor.author | Yocum, Charles F. | en_US |
dc.date.accessioned | 2006-09-08T21:32:41Z | |
dc.date.available | 2006-09-08T21:32:41Z | |
dc.date.issued | 1989-12 | en_US |
dc.identifier.citation | Hoganson, Curtis W.; Ghanotakis, Demetrios F.; Babcock, Gerald T.; Yocum, Charles F.; (1989). "Mn 2+ reduces Y z + in manganese-depleted Photosystem II preparations." Photosynthesis Research 22(3): 285-293. <http://hdl.handle.net/2027.42/43534> | en_US |
dc.identifier.issn | 0166-8595 | en_US |
dc.identifier.issn | 1573-5079 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/43534 | |
dc.description.abstract | Manganese in the oxygen-evolving complex is a physiological electron donor to Photosystem II. PS II depleted of manganese may oxidize exogenous reductants including benzidine and Mn 2+ . Using flash photolysis with electron spin resonance detection, we examined the room-temperature reaction kinetics of these reductants with Y z + , the tyrosine radical formed in PS II membranes under illumination. Kinetics were measured with membranes that did or did not contain the 33 kDa extrinsic polypeptide of PS II, whose presence had no effect on the reaction kinetics with either reductant. The rate of Y z + reduction by benzidine was a linear function of benzidine concentration. The rate of Y z + reduction by Mn 2+ at pH 6 increased linearly at low Mn 2+ concentrations and reached a maximum at the Mn 2+ concentrations equal to several times the reaction center concentration. The rate was inhibited by K + , Ca 2+ and Mg 2+ . These data are described by a model in which negative charge on the membrane causes a local increase in the cation concentration. The rate of Y z + reduction at pH 7.5 was biphasic with a fast 400 μs phase that suggests binding of Mn 2+ near Y z + at a site that may be one of the native manganese binding sites. | en_US |
dc.format.extent | 698833 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Kluwer Academic Publishers; Springer Science+Business Media | en_US |
dc.subject.other | Tyrosine Free Radical | en_US |
dc.subject.other | Plant Physiology | en_US |
dc.subject.other | Life Sciences | en_US |
dc.subject.other | Photosystem II | en_US |
dc.subject.other | Oxygen Evolution | en_US |
dc.subject.other | Manganese Oxidation | en_US |
dc.subject.other | Electron Transfer Kinetics | en_US |
dc.title | Mn 2+ reduces Y z + in manganese-depleted Photosystem II preparations | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Division of Biological Sciences, the University of Michigan, 48109-1055, Ann Arbor, MI, U.S.A.; Department of Chemistry, University of Crete, Ihraklion, Crete, Greece | en_US |
dc.contributor.affiliationum | Division of Biological Sciences, the University of Michigan, 48109-1055, Ann Arbor, MI, U.S.A. | en_US |
dc.contributor.affiliationother | Department of Chemistry, Michigan State University, 48824-1322, East Lansing, MI, U.S.A.; Department of Biochemistry and Biophysics, Chalmers Institute of Technology, S-41296, Goteborg, Sweden | en_US |
dc.contributor.affiliationother | Department of Chemistry, Michigan State University, 48824-1322, East Lansing, MI, U.S.A. | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 24424817 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/43534/1/11120_2004_Article_BF00048306.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/BF00048306 | en_US |
dc.identifier.source | Photosynthesis Research | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.