NMR paramagnetic relaxation enhancements due to manganese in the S 0 and S 2 states of Photosystem II-enriched membrane fragments and in the detergent-solubilized Photosystem II complex
Bovet, Jean-Marc; Park, Eun-Jung; Sharp, Robert R.
1993-01
Citation
Bovet, Jean-Marc; Park, Eun-Jung; Sharp, Robert R.; (1993). "NMR paramagnetic relaxation enhancements due to manganese in the S 0 and S 2 states of Photosystem II-enriched membrane fragments and in the detergent-solubilized Photosystem II complex." Photosynthesis Research 38(3): 347-354. <http://hdl.handle.net/2027.42/43536>
Abstract
The NMR paramagnetic relaxation enhancement (NMR-PRE) produced in the solvent proton resonance by manganese in the S 0 and S 2 states of the oxygen evolving center (OEC) has been recorded for three Photosystem II (PS II)-enriched preparations: (1) PS II-enriched thylakoid membrane fragments (TMF-2 particles); (2) salt-washed (2M NaCl) TMF-2 particles; and (3) the octylglucopyranoside (OGP)-solubilized PS II complex. The second and third preparations, but not the first, are depleted of the peripheral 17 and 23 kD polypeptides associated with the OEC. It has been proposed that depletion of these polypeptides increases the exposure of OEC manganese to the aqueous phase. The NMR-PRE response measures the quantity (T 1m +τ m ) -1 , where T 1m is the spin relaxation time and τ m is the mean residence time with respect to chemical exchange reactions of solvent protons in the manganese coordination sphere, and, thus, the NMR-PRE provides a direct measure of the solvent proton chemical exchange rate constant τ m -1 . This study tested whether the 17 and 23 kD polypeptides shield the OEC from the solvent phase and whether their depletion enhances the S 2 and S 0 NMR-PRE signals by removing a kinetic barrier to the solvent proton chemical exchange reaction. The amplitude of the S 2 NMR-PRE signal, measured in its chemical exchange-limited regime (τ m >T 1m ), is slightly decreased, rather than increased, in preparations (2) and (3) relative to (1), indicating that removal of the 17 and 23 kD polypeptides slightly slows, rather than accelerates, the rate-limiting steps of the solvent proton chemical exchange reactions. In addition, the lifetime of the S 2 state was shortened several-fold in the solubilized PS II complex and in salt-washed TMF-2 membranes relative to untreated TMF-2 control samples. The S 0 NMR-PRE signal, which is present in TMF-2 suspensions, was not detected in suspensions of the solubilized PS II complex, even though these samples contained high concentrations of active manganese centers (approximately double those of the TMF-2 control) and exhibited an S 2 NMR-PRE signal of comparable amplitude to that of the TMF-2 preparation. These results suggest that the 17 and 23 kD extrinsic polypeptides do not shield the NMR-visible water binding site in the OEC from the aqueous phase, although their removal substantially alters the proton relaxation efficiency by shortening T 1m .Publisher
Kluwer Academic Publishers; Springer Science+Business Media
ISSN
0166-8595 1573-5079
Other DOIs
PMID
24317989
Types
Article
Metadata
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