Comparison of gene expression under in vitro and ex vitro conditions during rooting of grape cuttings through mRNA differential display
dc.contributor.author | Thomas, Pious | en_US |
dc.contributor.author | Schiefelbein, John W. | en_US |
dc.date.accessioned | 2006-09-11T13:55:38Z | |
dc.date.available | 2006-09-11T13:55:38Z | |
dc.date.issued | 2005-01 | en_US |
dc.identifier.citation | Thomas, Pious; Schiefelbein, John W.; (2005). "Comparison of gene expression under in vitro and ex vitro conditions during rooting of grape cuttings through mRNA differential display." Plant Cell, Tissue and Organ Culture 80(1): 105-109. <http://hdl.handle.net/2027.42/43848> | en_US |
dc.identifier.issn | 1573-5044 | en_US |
dc.identifier.issn | 0167-6857 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/43848 | |
dc.description.abstract | An mRNA differential display (DD) analysis during rooting in grape cuttings was carried out to determine whether gene expression patterns differed under in vitro and ex vitro conditions. The four tissue samples for differential display and subsequent Northern hybridization analyses included control stem tissue from in vitro and ex vitro sources, microcuttings planted in MS based in vitro rooting medium and softwood cuttings planted in ex vitro soil medium, both collected 48 h after planting. DD autoradiographs showed gross similarity in banding pattern between in vitro and ex vitro stem tissue, whether constitutive or induced. Northern blot analysis of a few bands that appeared to be differentials did not indicate them as true positives. The results suggested that gene expression pattern during physiological processes such as rooting may be identical under in vitro and ex vitro conditions. | en_US |
dc.format.extent | 259579 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Kluwer Academic Publishers; Springer-Verlag | en_US |
dc.subject.other | Life Sciences | en_US |
dc.subject.other | Plant Sciences | en_US |
dc.subject.other | Adventitious Rooting | en_US |
dc.subject.other | Gene Expression | en_US |
dc.subject.other | In Vitro | en_US |
dc.subject.other | MRNA Differential Display | en_US |
dc.subject.other | Stem Cutting | en_US |
dc.subject.other | Vitis Vinifera | en_US |
dc.subject.other | Plant Physiology | en_US |
dc.title | Comparison of gene expression under in vitro and ex vitro conditions during rooting of grape cuttings through mRNA differential display | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI, 48109, USA | en_US |
dc.contributor.affiliationother | Division of Biotechnology, Indian Institute of Horticultural Research, Hessarghatta Lake, Bangalore, 560 089, India | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/43848/1/11240_2004_Article_5790.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/s11240-004-5790-y | en_US |
dc.identifier.source | Plant Cell, Tissue and Organ Culture | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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