Transgenic Zebrafish for Studying Nervous System Development and Regeneration
dc.contributor.author | Dai, Xinhua | en_US |
dc.contributor.author | Hankin, M. | en_US |
dc.contributor.author | Goldman, Daniel J. | en_US |
dc.contributor.author | Li, Z. | en_US |
dc.contributor.author | Ding, Jun | en_US |
dc.date.accessioned | 2006-09-11T13:56:16Z | |
dc.date.available | 2006-09-11T13:56:16Z | |
dc.date.issued | 2001-02 | en_US |
dc.identifier.citation | Goldman, D.; Hankin, M.; Li, Z.; Dai, X.; Ding, J.; (2001). "Transgenic Zebrafish for Studying Nervous System Development and Regeneration." Transgenic Research 10(1): 21-33. <http://hdl.handle.net/2027.42/43857> | en_US |
dc.identifier.issn | 1573-9368 | en_US |
dc.identifier.issn | 0962-8819 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/43857 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=11252380&dopt=citation | en_US |
dc.description.abstract | α1 tubulin gene expression is induced in the developing and regenerating CNS of vertebrates. Therefore, α1 tubulin gene expression may serve as a good probe for mechanisms underlying CNS development and regeneration. One approach to identify these mechanisms is to work backwards from the genome. This requires identification of α1 tubulin DNA sequences that mediate its developmental and regeneration-dependent expression pattern. Therefore, we generated transgenic zebrafish harboring a fragment of the α1 tubulin gene driving green fluorescent protein expression (GFP). In these fish, and similar to the endogenous gene, transgene expression was dramatically induced in the developing and regenerating nervous system. Although transgene expression generally declined during maturation of the nervous system, robust GFP expression was maintained in progenitor cells in the retinal periphery, lining brain ventricles and surrounding the central canal of the spinal cord. When these cells were cultured in vitro they divided and gave rise to new neurons. We also show that optic nerve crush in adult fish re-induced transgene expression in retinal ganglion cells. These studies identified a relatively small region of the α1 tubulin promoter that mediates its regulated expression pattern in developing and adult fish. This promoter will be extremely useful to investigators interested in targeting gene expression to the developing or regenerating nervous system. As adult transgenic fish maintain transgene expression in neural progenitors, these fish also provide a valuable resource of labeled adult neural progenitor cells that can be studied in vivo or in vitro . Finally, these fish should provide a unique in vivo system for investigating mechanisms mediating CNS development and regeneration. | en_US |
dc.format.extent | 305777 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Kluwer Academic Publishers-Plenum Publishers; Kluwer Academic Publishers ; Springer Science+Business Media | en_US |
dc.subject.other | Biomedicine | en_US |
dc.subject.other | Human Genetics | en_US |
dc.subject.other | Animal Anatomy / Morphology / Histology | en_US |
dc.subject.other | α1 Tubulin | en_US |
dc.subject.other | Gene Expression | en_US |
dc.subject.other | Neural Stem Cells | en_US |
dc.subject.other | Optic Nerve Regeneration | en_US |
dc.subject.other | Zebrafish | en_US |
dc.subject.other | Nervous System Development | en_US |
dc.subject.other | Plant Sciences | en_US |
dc.title | Transgenic Zebrafish for Studying Nervous System Development and Regeneration | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Genetics | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Mental Health Research Institute and Department of Biological Chemistry, University of Michigan, 205 Zina Pitcher Place, Ann Arbor, MI, 48109 | en_US |
dc.contributor.affiliationum | Mental Health Research Institute and Department of Biological Chemistry, University of Michigan, 205 Zina Pitcher Place, Ann Arbor, MI, 48109; Department of Anatomy and Neurobiology, Medical College of Ohio, Toledo, OH, 43614 | en_US |
dc.contributor.affiliationum | Mental Health Research Institute and Department of Biological Chemistry, University of Michigan, 205 Zina Pitcher Place, Ann Arbor, MI, 48109; Parke Davis, Ann Arbor, MI, 48109 | en_US |
dc.contributor.affiliationum | Mental Health Research Institute and Department of Biological Chemistry, University of Michigan, 205 Zina Pitcher Place, Ann Arbor, MI, 48109 | en_US |
dc.contributor.affiliationum | Mental Health Research Institute and Department of Biological Chemistry, University of Michigan, 205 Zina Pitcher Place, Ann Arbor, MI, 48109 | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 11252380 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/43857/1/11248_2004_Article_281333.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1023/A:1008998832552 | en_US |
dc.identifier.source | Transgenic Research | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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