Genetic control of quantitative variation in carbonic anhydrase isozymes of mammals. I. The concentration within single erythrocytes of the mouse
dc.contributor.author | Hsu, Sun S. | en_US |
dc.contributor.author | Tashian, Richard E. | en_US |
dc.date.accessioned | 2006-09-11T14:19:28Z | |
dc.date.available | 2006-09-11T14:19:28Z | |
dc.date.issued | 1977-10 | en_US |
dc.identifier.citation | Hsu, Sun S.; Tashian, Richard E.; (1977). "Genetic control of quantitative variation in carbonic anhydrase isozymes of mammals. I. The concentration within single erythrocytes of the mouse." Biochemical Genetics 15 (9-10): 885-896. <http://hdl.handle.net/2027.42/44126> | en_US |
dc.identifier.issn | 0006-2928 | en_US |
dc.identifier.issn | 1573-4927 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/44126 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=412493&dopt=citation | en_US |
dc.description.abstract | The levels of the carbonic anhydrase isozymes (CA and CA II) in single erythrocytes of DBA/2J mice were assayed by measuring the specific immunofluorescence of CA I and CA II with a microspectrofluorometric technique. Measurements of 100 randomly selected cells showed a range (in relative fluorescence units) of 21–52 (mean 31.3±7.5) for CA I and 30–80 (mean 45.6±10.7) for CA II. The CA II/CA I ratio of the means obtained by the single-cell fluorescence assay was similar to the ratios obtained for the two isozymes from hemolysates of DBA/2J mice using an immunodiffusion assay. The influence of cell age on the variation in carbonic anhydrase levels was determined by separating red cells into several fractions by a gravity sedimentation procedure. The younger cells showed higher levels of CA I and CA II than the older cells; however, the extensive overlap in the variability between the cells from the different fractions indicated that although cell age was contributing to the overall heterogeneity, its influence was not pronounced. | en_US |
dc.format.extent | 683295 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Kluwer Academic Publishers-Plenum Publishers; Plenum Publishing Corporation ; Springer Science+Business Media | en_US |
dc.subject.other | Mouse Red Cells | en_US |
dc.subject.other | Medical Microbiology | en_US |
dc.subject.other | Biomedicine | en_US |
dc.subject.other | Human Genetics | en_US |
dc.subject.other | Biochemistry, General | en_US |
dc.subject.other | Zoology | en_US |
dc.subject.other | Carbonic Anhydrases I and II | en_US |
dc.subject.other | Immunofluorescence | en_US |
dc.subject.other | Microspectrofluorometry | en_US |
dc.title | Genetic control of quantitative variation in carbonic anhydrase isozymes of mammals. I. The concentration within single erythrocytes of the mouse | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Human Genetics, University of Michigan Medical School, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Department of Human Genetics, University of Michigan Medical School, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 412493 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/44126/1/10528_2004_Article_BF00483984.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/BF00483984 | en_US |
dc.identifier.source | Biochemical Genetics | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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