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Improved method for the identification of the fluoride-resistant plasmacholinesterase genotypes
dc.contributor.authorKothary, Sarla P.en_US
dc.contributor.authorZsigmond, Elemér K.en_US
dc.contributor.authorFlynn, Kathleen B.en_US
dc.date.accessioned2006-09-11T14:20:20Z
dc.date.available2006-09-11T14:20:20Z
dc.date.issued1980-10en_US
dc.identifier.citationZsigmond, E. K.; Flynn, Kathleen B.; Kothary, S. P.; (1980). "Improved method for the identification of the fluoride-resistant plasmacholinesterase genotypes." Biochemical Genetics 18 (9-10): 861-867. <http://hdl.handle.net/2027.42/44136>en_US
dc.identifier.issn0006-2928en_US
dc.identifier.issn1573-4927en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/44136
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=7225083&dopt=citationen_US
dc.description.abstractThis investigation was prompted by the findings that (1) dibucaine-resistant homozygotes and heterozygotes for plasmacholinesterase also exhibit resistance to fluoride inhibition, (2) the differentiation of dibucaine-resistant from the fluoride-resistant genotypes is ambiguous with the method of Harris and Whittaker, (3) the plasmacholinesterase inhibition by Na fluoride (FN) is markedly influenced by the temperature. Therefore, we modified their method by increasing (1) the temperature of the reaction from 25C to 37C and (2) the concentration of Na fluoride from 5.0×10 −5 m to 2.5×10 −4 m . With this method, genetically normal individuals have a mean FN± sd =77.0±3.22 while atypical dibucaine-resistant homozygotes have a mean FN± sd =43.0±10.0 and atypical dibucaine-resistant heterozygotes 67.0±5.37. Since a linear correlation was observed between DN and FN by our new method, a fluoride number 2 sd lower than the predicted FN from the DN can distinctly identify the fluoride-resistant plasmacholinesterase genotype E 1 f .en_US
dc.format.extent317417 bytes
dc.format.extent3115 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherKluwer Academic Publishers-Plenum Publishers; Plenum Publishing Corporation ; Springer Science+Business Mediaen_US
dc.subject.otherEnzyme Assayen_US
dc.subject.otherZoologyen_US
dc.subject.otherBiomedicineen_US
dc.subject.otherHuman Geneticsen_US
dc.subject.otherMedical Microbiologyen_US
dc.subject.otherBiochemistry, Generalen_US
dc.subject.otherFN Determinationen_US
dc.subject.otherPlasmacholinesteraseen_US
dc.subject.otherE 1 F Genotypeen_US
dc.subject.otherModified FNen_US
dc.titleImproved method for the identification of the fluoride-resistant plasmacholinesterase genotypesen_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelNatural Resources and Environmenten_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbsecondlevelEcology and Evolutionary Biologyen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Anesthesiology and Anesthesia Research Laboratories, University of Michigan Medical Center, 48109, Ann Arbor, Michigan; Department of Anesthesiology, University of Illinois Medical Center, 60612, Chicago, Illinoisen_US
dc.contributor.affiliationumDepartment of Anesthesiology and Anesthesia Research Laboratories, University of Michigan Medical Center, 48109, Ann Arbor, Michiganen_US
dc.contributor.affiliationumDepartment of Anesthesiology and Anesthesia Research Laboratories, University of Michigan Medical Center, 48109, Ann Arbor, Michiganen_US
dc.contributor.affiliationumcampusAnn Arboren_US
dc.identifier.pmid7225083en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/44136/1/10528_2004_Article_BF00500119.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1007/BF00500119en_US
dc.identifier.sourceBiochemical Geneticsen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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