Studies of enzyme polymorphisms in the Kamuela population of Drosophila mercatorum . II. Evaluation of glycolytic intermediates
dc.contributor.author | Clark, R. L. | en_US |
dc.contributor.author | Brewer, George J. | en_US |
dc.contributor.author | Sing, Charles F. | en_US |
dc.date.accessioned | 2006-09-11T14:20:53Z | |
dc.date.available | 2006-09-11T14:20:53Z | |
dc.date.issued | 1982-12 | en_US |
dc.identifier.citation | Clark, R. L.; Brewer, G. J.; Sing, C. F.; (1982). "Studies of enzyme polymorphisms in the Kamuela population of Drosophila mercatorum . II. Evaluation of glycolytic intermediates." Biochemical Genetics 20 (11-12): 1085-1103. <http://hdl.handle.net/2027.42/44142> | en_US |
dc.identifier.issn | 0006-2928 | en_US |
dc.identifier.issn | 1573-4927 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/44142 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=7165691&dopt=citation | en_US |
dc.description.abstract | A simple and effective cryogenic procedure for the extraction of glycolytic intermediates from whole Drosophila has been developed. This procedure gives consistent results when a measure (µM/liter/OD 260 ) is adopted which corrects for differences in extraction efficiency. Using this measure and a homozygous strain of D. mercatorum , there are no significant differences among extracts for the levels of any of the 15 glycolytic intermediate or energy molecules considered. The profile of means is consistent across experimental designs and instrument types. Coefficients of variation are well below 50% for most variables. The methodology presented has the statistical power to detect a mean change of 10 to 50% using an experimental design which requires as few as 32 observations. The estimated energy charge for resting Drosophila from these studies is the expected value of 0.86. | en_US |
dc.format.extent | 898891 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Kluwer Academic Publishers-Plenum Publishers; Plenum Publishing Corporation ; Springer Science+Business Media | en_US |
dc.subject.other | Drosophila Mercatorum | en_US |
dc.subject.other | Human Genetics | en_US |
dc.subject.other | Biomedicine | en_US |
dc.subject.other | Medical Microbiology | en_US |
dc.subject.other | Biochemistry, General | en_US |
dc.subject.other | Zoology | en_US |
dc.subject.other | Glycolytic Intermediates | en_US |
dc.subject.other | Energy Charge | en_US |
dc.title | Studies of enzyme polymorphisms in the Kamuela population of Drosophila mercatorum . II. Evaluation of glycolytic intermediates | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Human Genetics, University of Michigan, 48109, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Department of Human Genetics, University of Michigan, 48109, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Department of Human Genetics, University of Michigan, 48109, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 7165691 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/44142/1/10528_2004_Article_BF00498934.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/BF00498934 | en_US |
dc.identifier.source | Biochemical Genetics | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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