Cross-reactions among carbonic anhydrases I, II, and III studied by binding tests and with monoclonal antibodies
dc.contributor.author | Tashian, Richard E. | en_US |
dc.contributor.author | Erickson, Robert P. | en_US |
dc.contributor.author | Hewett-Emmett, David | en_US |
dc.contributor.author | Claflin, J. Latham | en_US |
dc.contributor.author | Kay, Gary | en_US |
dc.date.accessioned | 2006-09-11T14:21:04Z | |
dc.date.available | 2006-09-11T14:21:04Z | |
dc.date.issued | 1982-08 | en_US |
dc.identifier.citation | Erickson, Robert P.; Kay, Gary; Hewett-Emmett, David; Tashian, Richard E.; Claflin, J. Latham; (1982). "Cross-reactions among carbonic anhydrases I, II, and III studied by binding tests and with monoclonal antibodies." Biochemical Genetics 20 (7-8): 809-819. <http://hdl.handle.net/2027.42/44144> | en_US |
dc.identifier.issn | 0006-2928 | en_US |
dc.identifier.issn | 1573-4927 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/44144 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=6814422&dopt=citation | en_US |
dc.description.abstract | Cross-reactions among carbonic anhydrases (CAs) I, II, and III were studied using a variety of antisera: (1) a rabbit antiserum to bovine CA III, (2) mouse antisera to human CA I, CA II, and CA III; and (3) five monoclonal antibodies prepared by the hybridoma technique using splenocytes from a mouse immunized with human CAs I and II and bovine CA III. Cross-reactions between CAs were readily found by binding assays using these antisera. Human CA I, but not human CA II, inhibited the reaction of the rabbit anti-CA III with its homologous antigen. Mouse antisera to CA I or CA II bound the homologous I or II with nearly as great efficiency as the autologous isozyme and sometimes weakly bound CA III. Mouse antisera to CA III frequently bound CA I or II. These cross-reactions were confirmed by the first use of hybridoma-prepared, monoclonal antibodies to CAs. The mouse monoclonal antibodies to CA isozymes varied in the amount of cross-reactivity among I, II, and III: at one extreme, one monoclonal was highly specific for the autologous CA III; at the other extreme, one monoclonal weakly reacted with some examples of CAs I, II, and III. | en_US |
dc.format.extent | 568460 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Kluwer Academic Publishers-Plenum Publishers; Plenum Publishing Corporation ; Springer Science+Business Media | en_US |
dc.subject.other | Isozymes | en_US |
dc.subject.other | Biomedicine | en_US |
dc.subject.other | Human Genetics | en_US |
dc.subject.other | Biochemistry, General | en_US |
dc.subject.other | Zoology | en_US |
dc.subject.other | Carbonic Anhydrase | en_US |
dc.subject.other | Immunological Cross-reactions | en_US |
dc.subject.other | Monoclonal Antibodies | en_US |
dc.subject.other | Protein Evolution | en_US |
dc.subject.other | Medical Microbiology | en_US |
dc.title | Cross-reactions among carbonic anhydrases I, II, and III studied by binding tests and with monoclonal antibodies | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Human Genetics, University of Michigan Medical School, 48109, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Department of Human Genetics, University of Michigan Medical School, 48109, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Department of Human Genetics, University of Michigan Medical School, 48109, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Department of Human Genetics, University of Michigan Medical School, 48109, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Department of Microbiology and Immunology, University of Michigan Medical School, 48109, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 6814422 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/44144/1/10528_2004_Article_BF00483975.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/BF00483975 | en_US |
dc.identifier.source | Biochemical Genetics | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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