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Pleiotropic mutants of Chinese hamster cells with altered cytidine 5′-triphosphate synthetase

dc.contributor.authorLi, I-Chianen_US
dc.contributor.authorChu, Ernest H. Y.en_US
dc.contributor.authorMcLaren, John D.en_US
dc.contributor.authorLamb, Barbara J.en_US
dc.date.accessioned2006-09-11T14:21:33Z
dc.date.available2006-09-11T14:21:33Z
dc.date.issued1984-08en_US
dc.identifier.citationChu, Ernest H. Y.; McLaren, John D.; Li, I-Chian; Lamb, Barbara; (1984). "Pleiotropic mutants of Chinese hamster cells with altered cytidine 5′-triphosphate synthetase." Biochemical Genetics 22 (7-8): 701-715. <http://hdl.handle.net/2027.42/44150>en_US
dc.identifier.issn0006-2928en_US
dc.identifier.issn1573-4927en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/44150
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=6497832&dopt=citationen_US
dc.description.abstractFollowing chemical mutagenesis and multiple-step indirect selection, four clones of Chinese hamster V79 cells were isolated which exhibited auxotrophy for thymidine, deoxycytidine, or deoxyuridine but not for cytidine or uridine. All were resistant to uridine, 3-deazauridine, 5-fluorouridine, thymidine, and cytosine arabinoside at concentrations that were toxic to wild-type V79 cells. The cytidine 5′-triphosphate (CTP) and deoxycytidine 5′-triphosphate (dCTP) pools in the mutants were expanded, but the uridine 5′-triphosphate (UTP) pool either decreased or remained unchanged relative to the wild-type level. Furthermore, since the parental cells appear to be deficient in dCMP deaminase activity and CTP (or one of its metabolites) has been shown to inhibit uridine 5′-diphosphate (UDP) reduction, an elevated CTP level should lead to the observed thymidine auxotrophy. It also explains the joint resistance of mutant clones to thymidine and cytosine arabinoside. The change in the ratio of intracellular dCTP to thymidine 5′-triphosphate (dTTP) may be responsible for the elevation in the rates of spontaneous mutations in these mutants.en_US
dc.format.extent775964 bytes
dc.format.extent3115 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherKluwer Academic Publishers-Plenum Publishers; Plenum Publishing Corporation ; Springer Science+Business Mediaen_US
dc.subject.otherHuman Geneticsen_US
dc.subject.otherCytidine 5′-Triphosphate Synthetaseen_US
dc.subject.otherNucleotide Poolsen_US
dc.subject.otherBiomedicineen_US
dc.subject.otherMedical Microbiologyen_US
dc.subject.otherBiochemistry, Generalen_US
dc.subject.otherZoologyen_US
dc.subject.otherChinese Hamster Cellsen_US
dc.subject.otherPyrimidine Metabolismen_US
dc.subject.otherK M Mutantsen_US
dc.subject.otherFeedback Inhibitionen_US
dc.titlePleiotropic mutants of Chinese hamster cells with altered cytidine 5′-triphosphate synthetaseen_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelMolecular, Cellular and Developmental Biologyen_US
dc.subject.hlbsecondlevelNatural Resources and Environmenten_US
dc.subject.hlbsecondlevelEcology and Evolutionary Biologyen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumLawrence D. Buhl Center for Human Genetics, Department of Human Genetics, University of Michigan Medical School, 48109, Ann Arbor, Michiganen_US
dc.contributor.affiliationumLawrence D. Buhl Center for Human Genetics, Department of Human Genetics, University of Michigan Medical School, 48109, Ann Arbor, Michiganen_US
dc.contributor.affiliationumLawrence D. Buhl Center for Human Genetics, Department of Human Genetics, University of Michigan Medical School, 48109, Ann Arbor, Michiganen_US
dc.contributor.affiliationumLawrence D. Buhl Center for Human Genetics, Department of Human Genetics, University of Michigan Medical School, 48109, Ann Arbor, Michiganen_US
dc.contributor.affiliationumcampusAnn Arboren_US
dc.identifier.pmid6497832en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/44150/1/10528_2004_Article_BF00485854.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1007/BF00485854en_US
dc.identifier.sourceBiochemical Geneticsen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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