Amino acid sequence of the active site of human serum cholinesterase from usual, atypical, and atypical-silent genotypes
dc.contributor.author | Lockridge, Oksana | en_US |
dc.contributor.author | Du, Bert N. | en_US |
dc.date.accessioned | 2006-09-11T14:21:50Z | |
dc.date.available | 2006-09-11T14:21:50Z | |
dc.date.issued | 1986-06 | en_US |
dc.identifier.citation | Lockridge, Oksana; Du, Bert N.; (1986). "Amino acid sequence of the active site of human serum cholinesterase from usual, atypical, and atypical-silent genotypes." Biochemical Genetics 24 (5-6): 485-498. <http://hdl.handle.net/2027.42/44153> | en_US |
dc.identifier.issn | 0006-2928 | en_US |
dc.identifier.issn | 1573-4927 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/44153 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=3741370&dopt=citation | en_US |
dc.description.abstract | Active-site tryptic peptides were isolated from three genetic types of human serum cholinesterase. The active-site peptide was identified by labeling the active-site serine with [ 3 H] diisopropylfluorophosphate. Peptides were purified by high-performance liquid chromatography. Amino acid composition and sequence analysis showed that the peptide from the usual genotype contained 29 residues with the sequence Ser-Val-Thr-Leu-Phe-Gly-Glu-Ser-Ala-Gly-Ala-Ala-Ser-Val-Ser-Leu-His-Leu-Leu-Ser-Pro-Gly-Ser-His-Ser-Leu-Phe-Thr-Arg. The active-site serine was the eighth residue from the N- terminal. The peptide containing the active-site serine from the atypical genotype contained 22 residues with the sequence Ser-Val-Thr-Leu-Phe-Gly-Glu-Ser-Ala-Gly-Ala-Ala-Ser-Val-Ser-Leu-His-Leu-Leu-Ser-Pro-Gly. The peptide from the atypical-silent genotype contained eight residues with the sequence Gly-Glu-Ser-Ala-Gly-Ala-Ala-Ser. Thus, the sequences of the atypical and atypical-silent active-site peptides were identical to the corresponding portions of the usual peptide. | en_US |
dc.format.extent | 735864 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Kluwer Academic Publishers-Plenum Publishers; Plenum Publishing Corporation ; Springer Science+Business Media | en_US |
dc.subject.other | Active-site Sequence | en_US |
dc.subject.other | Medical Microbiology | en_US |
dc.subject.other | Biomedicine | en_US |
dc.subject.other | Human Genetics | en_US |
dc.subject.other | Biochemistry, General | en_US |
dc.subject.other | Zoology | en_US |
dc.subject.other | Cholinesterase | en_US |
dc.title | Amino acid sequence of the active site of human serum cholinesterase from usual, atypical, and atypical-silent genotypes | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Pharmacology Department, Medical Science I, M6322, University of Michigan, 48109-0010, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Pharmacology Department, Medical Science I, M6322, University of Michigan, 48109-0010, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 3741370 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/44153/1/10528_2004_Article_BF00499101.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/BF00499101 | en_US |
dc.identifier.source | Biochemical Genetics | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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