Variation in coding exons of two electrophoretic alleles at the pigtail macaque carbonic anhydrase I locus as determined by direct, double-stranded sequencing of polymerase chain reaction (PCR) products
dc.contributor.author | Bergenhem, Nils C. H. | en_US |
dc.contributor.author | Venta, Patrick J. | en_US |
dc.contributor.author | Hopkins, Penelope J. | en_US |
dc.contributor.author | Tashian, Richard E. | en_US |
dc.date.accessioned | 2006-09-11T14:22:13Z | |
dc.date.available | 2006-09-11T14:22:13Z | |
dc.date.issued | 1992-06 | en_US |
dc.identifier.citation | Bergenhem, Nils C. H.; Venta, Patrick J.; Hopkins, Penelope J.; Tashian, Richard E.; (1992). "Variation in coding exons of two electrophoretic alleles at the pigtail macaque carbonic anhydrase I locus as determined by direct, double-stranded sequencing of polymerase chain reaction (PCR) products." Biochemical Genetics 30 (5-6): 279-287. <http://hdl.handle.net/2027.42/44158> | en_US |
dc.identifier.issn | 1573-4927 | en_US |
dc.identifier.issn | 0006-2928 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/44158 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=1616482&dopt=citation | en_US |
dc.description.abstract | Two, electrophoretically distinct, forms of carbonic anhydrase I (CA Ia and CA Ib) are found at high polymorphic frequencies in red cells of natural populations of pigtail macaques, Macaca nemestrina , from southeast Asia. By use of the polymerase chain reaction, exons of the CA I gene were amplified from homozygous ( a/a, b/b ) and heterozygous ( a/b ) animals. Direct sequencing of the amplified DNA from four animals revealed differences between the a and the b electrophoretic alleles ranging from three to six nucleotides, and from one to three differences within each allele. These results indicate a greater genetic variability at the CA I locus in this macaque species than previously realized. | en_US |
dc.format.extent | 498917 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Kluwer Academic Publishers-Plenum Publishers; Plenum Publishing Corporation ; Springer Science+Business Media | en_US |
dc.subject.other | Polymerase Chain Reaction | en_US |
dc.subject.other | Biochemistry, General | en_US |
dc.subject.other | Biomedicine | en_US |
dc.subject.other | Human Genetics | en_US |
dc.subject.other | Medical Microbiology | en_US |
dc.subject.other | Zoology | en_US |
dc.subject.other | Macaca Nemestrina | en_US |
dc.subject.other | Carbonic Anhydrase I Locus | en_US |
dc.subject.other | DNA Sequencing | en_US |
dc.subject.other | Allelic Variation | en_US |
dc.title | Variation in coding exons of two electrophoretic alleles at the pigtail macaque carbonic anhydrase I locus as determined by direct, double-stranded sequencing of polymerase chain reaction (PCR) products | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Human Genetics, University of Michigan Medical School, 48109-0618, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Department of Human Genetics, University of Michigan Medical School, 48109-0618, Ann Arbor, Michigan; Small Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, 48824-1314, East Lansing, Michigan | en_US |
dc.contributor.affiliationum | Department of Human Genetics, University of Michigan Medical School, 48109-0618, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Department of Human Genetics, University of Michigan Medical School, 48109-0618, Ann Arbor, Michigan; Department of Molecular and Experimental Medicine, Research Institute of Scripps Clinic, 10666 North Torrey Pines Road, 92037, La Jolla, California | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 1616482 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/44158/1/10528_2004_Article_BF00553755.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/BF00553755 | en_US |
dc.identifier.source | Biochemical Genetics | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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