Proboscidean DNA from Museum and Fossil Specimens: An Assessment of Ancient DNA Extraction and Amplification Techniques
dc.contributor.author | Yang, Hong | en_US |
dc.contributor.author | Golenberg, Edward M. | en_US |
dc.contributor.author | Shoshani, Jeheskel | en_US |
dc.date.accessioned | 2006-09-11T14:22:36Z | |
dc.date.available | 2006-09-11T14:22:36Z | |
dc.date.issued | 1997-06 | en_US |
dc.identifier.citation | Yang, Hong; Golenberg, Edward M.; Shoshani, Jeheskel; (1997). "Proboscidean DNA from Museum and Fossil Specimens: An Assessment of Ancient DNA Extraction and Amplification Techniques." Biochemical Genetics 35 (5-6): 165-179. <http://hdl.handle.net/2027.42/44162> | en_US |
dc.identifier.issn | 0006-2928 | en_US |
dc.identifier.issn | 1573-4927 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/44162 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=9332711&dopt=citation | en_US |
dc.description.abstract | Applications of reliable DNA extraction and amplification techniques to postmortem samples are critical to ancient DNA research. Commonly used methods for isolating DNA from ancient material were tested and compared using both soft tissue and bones from fossil and contemporary museum proboscideans. DNAs isolated using three principal methods served as templates in subsequent PCR amplifications, and the PCR products were directly sequenced. Authentication of the ancient origin of obtained nucleotide sequences was established by demonstrating reproducibility under a blind testing system and by phylogenetic analysis. Our results indicate that ancient samples may respond differently to extraction buffers or purification procedures, and no single method was universally successful. A CTAB buffer method, modified from plant DNA extraction protocols, was found to have the highest success rate. Nested PCR was shown to be a reliable approach to amplify ancient DNA templates that failed in primary amplification. | en_US |
dc.format.extent | 1047963 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Kluwer Academic Publishers-Plenum Publishers; Plenum Publishing Corporation ; Springer Science+Business Media | en_US |
dc.subject.other | Cetyltrimethylammonium Bromide Extraction | en_US |
dc.subject.other | Biomedicine | en_US |
dc.subject.other | Human Genetics | en_US |
dc.subject.other | Medical Microbiology | en_US |
dc.subject.other | Biochemistry, General | en_US |
dc.subject.other | Zoology | en_US |
dc.subject.other | Ancient DNA | en_US |
dc.subject.other | Nested Polymerase Chain Reaction | en_US |
dc.subject.other | Proboscideans | en_US |
dc.title | Proboscidean DNA from Museum and Fossil Specimens: An Assessment of Ancient DNA Extraction and Amplification Techniques | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biological Sciences, Wayne State University, Detroit, Michigan, 48202; Department of Human Genetics, University of Michigan Medical School, Ann Arbor, Michigan, 48109 | en_US |
dc.contributor.affiliationother | Department of Biological Sciences, Wayne State University, Detroit, Michigan, 48202 | en_US |
dc.contributor.affiliationother | Department of Biological Sciences, Wayne State University, Detroit, Michigan, 48202; Cranbrook Institute of Science, Bloomfield Hills, Michigan, 48304 | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 9332711 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/44162/1/10528_2004_Article_413527.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1023/A:1021902125382 | en_US |
dc.identifier.source | Biochemical Genetics | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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