Microdissection and microcloning of chromosomal alterations in human breast cancer
dc.contributor.author | Collins, Francis S. | en_US |
dc.contributor.author | Diamond, Austin | en_US |
dc.contributor.author | Guan, X. Y. | en_US |
dc.contributor.author | Trent, Jeffrey M. | en_US |
dc.contributor.author | Weber, Barbara L. | en_US |
dc.contributor.author | Zhang, Ji | en_US |
dc.contributor.author | Abel, Kenneth J. | en_US |
dc.contributor.author | Meltzer, Paul S. | en_US |
dc.date.accessioned | 2006-09-11T14:25:49Z | |
dc.date.available | 2006-09-11T14:25:49Z | |
dc.date.issued | 1995-02 | en_US |
dc.identifier.citation | Trent, Jeffrey M.; Weber, Barbara; Guan, X. Y.; Zhang, Ji; Collins, Francis; Abel, Ken; Diamond, Austin; Meltzer, Paul; (1995). "Microdissection and microcloning of chromosomal alterations in human breast cancer." Breast Cancer Research and Treatment 33(2): 95-102. <http://hdl.handle.net/2027.42/44200> | en_US |
dc.identifier.issn | 0167-6806 | en_US |
dc.identifier.issn | 1573-7217 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/44200 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=7749145&dopt=citation | en_US |
dc.description.abstract | The recognition of recurring sites of chromosome changes in malignancies has greatly facilitated the identification of genes implicated in the pathogenesis of human cancers. Based especially upon recent studies [1–4], it appears increasingly likely that a subset of recurring chromosome alterations will be recognized in human breast cancer. Currently recognized chromosome changes characterizing breast carcinoma include the recognition of cytologic features of gene amplification (e.g. double minutes [dmins] and homogeneously staining regions [HSRs]) [5–8]. As these and other chromosome regions are implicated in recurring abnormalities in breast cancer, it will become increasingly important to have band-or region-specific genomic libraries and probes in order to facilitate high resolution physical mapping and ultimately to clone breast cancer related genes [9]. Toward this end an important recent development in physical mapping has been the establishment of chromosome microdissection as a rapid and reproducible approach to rapidly isolate and characterize chromosome region-specific DNA, greatly facilitating the initial steps in positional cloning of disease-related genes [10–13]. In this brief report, we will highlight the application of chromosome microdissection to the generation of region-specific probes for both fluorescent in situ hybridization (FISH) and the generation of genomic microclone libraries. Additionally, efforts using this methodology to generate a microclone library encompassing the early onset breast/ovarian cancer (BRCA1) gene will be presented. | en_US |
dc.format.extent | 2651641 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Kluwer Academic Publishers; Springer Science+Business Media | en_US |
dc.subject.other | Oncology | en_US |
dc.subject.other | Medicine & Public Health | en_US |
dc.subject.other | Chromosomes | en_US |
dc.subject.other | Breast Cancer | en_US |
dc.subject.other | Chromosome Microdissection | en_US |
dc.subject.other | Physical Mapping | en_US |
dc.title | Microdissection and microcloning of chromosomal alterations in human breast cancer | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Obstetrics and Gynecology | en_US |
dc.subject.hlbsecondlevel | Oncology and Hematology | en_US |
dc.subject.hlbsecondlevel | Ophthalmology | en_US |
dc.subject.hlbsecondlevel | Internal Medicine and Specialties | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbsecondlevel | Otolaryngology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Internal Medicine and the Human Genome Center, University of Michigan, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationum | Department of Internal Medicine and the Human Genome Center, University of Michigan, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationum | Department of Internal Medicine and the Human Genome Center, University of Michigan, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationother | Laboratory of Cancer Genetics, National Center for Genome Research, National Institutes of Health, 9000 Rockville Pike, Bldg 49 Rm 4A22, 20892, Bethesda, MD, USA | en_US |
dc.contributor.affiliationother | Laboratory of Cancer Genetics, National Center for Genome Research, National Institutes of Health, 9000 Rockville Pike, Bldg 49 Rm 4A22, 20892, Bethesda, MD, USA | en_US |
dc.contributor.affiliationother | Laboratory of Cancer Genetics, National Center for Genome Research, National Institutes of Health, 9000 Rockville Pike, Bldg 49 Rm 4A22, 20892, Bethesda, MD, USA | en_US |
dc.contributor.affiliationother | Laboratory of Cancer Genetics, National Center for Genome Research, National Institutes of Health, 9000 Rockville Pike, Bldg 49 Rm 4A22, 20892, Bethesda, MD, USA | en_US |
dc.contributor.affiliationother | Laboratory of Cancer Genetics, National Center for Genome Research, National Institutes of Health, 9000 Rockville Pike, Bldg 49 Rm 4A22, 20892, Bethesda, MD, USA | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 7749145 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/44200/1/10549_2004_Article_BF00682717.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/BF00682717 | en_US |
dc.identifier.source | Breast Cancer Research and Treatment | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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