Insulin-like growth factor-I induces the phosphorylation and nuclear exclusion of forkhead transcription factors in human neuroblastoma cells
dc.contributor.author | Schwab, Tracy S. | en_US |
dc.contributor.author | Madison, B. B. | en_US |
dc.contributor.author | Grauman, A. R. | en_US |
dc.contributor.author | Feldman, Eva L. | en_US |
dc.date.accessioned | 2006-09-11T14:40:01Z | |
dc.date.available | 2006-09-11T14:40:01Z | |
dc.date.issued | 2005-08 | en_US |
dc.identifier.citation | Schwab, T. S.; Madison, B. B.; Grauman, A. R.; Feldman, E. L.; (2005). "Insulin-like growth factor-I induces the phosphorylation and nuclear exclusion of forkhead transcription factors in human neuroblastoma cells." Apoptosis 10(4): 831-840. <http://hdl.handle.net/2027.42/44353> | en_US |
dc.identifier.issn | 1360-8185 | en_US |
dc.identifier.issn | 1573-675X | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/44353 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=16133873&dopt=citation | en_US |
dc.description.abstract | Akt-mediated phosphorylation of forkhead transcription factors is linked to growth factor-stimulated cell survival. We investigated whether the survival activity of insulin-like growth factor-I (IGF-I) in SH-SY5Y human neuroblastoma (NBL) cells is associated with phosphorylation and/or localization changes in forkhead proteins. IGF-I induced phosphorylation of Erks (p42/p44), FKHR (FOXO1a) (Ser 253), FKHRL1 (FOXO3a) (Ser 256), and Akt (Ser 473). PI3-K inhibitor, LY294002, reduced IGF-I-stimulated phosphorylation of FKHR, FKHRL1, and Akt, but did not affect Erk phosphorylation. Using a GFP-FKHR construct, FKHR imported into the nucleus during growth factor withdrawal-induced apoptosis. In addition, IGF-I rescue from serum withdrawal-induced apoptosis is associated with a rapid export of GFP-FKHR into the cytoplasm. Leptomycin B, an inhibitor of Crm1-mediated nuclear export, decreased the level of FKHRL1 phosphorylation in the presence of IGF-I in vector and FKHR overexpressing cells, but had no effect on the phosphorylation status of FKHR. In addition, leptomycin B prevented IGF-I stimulated nuclear export of GFP-FKHR. These studies show IGF-I phosphorylation of FKHR and FKHRL1 via a PI3-K-dependent pathway in NBL cells. | en_US |
dc.format.extent | 733272 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Kluwer Academic Publishers; Springer Science + Business Media, Inc. | en_US |
dc.subject.other | Medicine & Public Health | en_US |
dc.subject.other | Cancer Research | en_US |
dc.subject.other | Virology | en_US |
dc.subject.other | Oncology | en_US |
dc.subject.other | Biochemistry, General | en_US |
dc.subject.other | Cell Biology | en_US |
dc.subject.other | FKHR | en_US |
dc.subject.other | FKHRL1 | en_US |
dc.subject.other | Forkhead | en_US |
dc.subject.other | IGF-I | en_US |
dc.subject.other | IGF-IR | en_US |
dc.subject.other | Neuroblastoma | en_US |
dc.title | Insulin-like growth factor-I induces the phosphorylation and nuclear exclusion of forkhead transcription factors in human neuroblastoma cells | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Radiology | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Neurology, University of Michigan, 4414 Kresge III, 200 Zina Pitcher Place, Ann Arbor, MI, 48109 | en_US |
dc.contributor.affiliationum | Department of Neurology, University of Michigan, 4414 Kresge III, 200 Zina Pitcher Place, Ann Arbor, MI, 48109 | en_US |
dc.contributor.affiliationum | Department of Neurology, University of Michigan, 4414 Kresge III, 200 Zina Pitcher Place, Ann Arbor, MI, 48109 | en_US |
dc.contributor.affiliationum | Department of Neurology, University of Michigan, 4414 Kresge III, 200 Zina Pitcher Place, Ann Arbor, MI, 48109 | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 16133873 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/44353/1/10495_2005_Article_429.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/s10495-005-0429-y | en_US |
dc.identifier.source | Apoptosis | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.