Caspase-3/CPP32-like activity is not sufficient to mediate apoptosis in an IL-2 dependent T cell line
dc.contributor.author | Vasilakos, John P. | en_US |
dc.contributor.author | Lynch, T. | en_US |
dc.contributor.author | Ghayur, T. | en_US |
dc.contributor.author | Giegel, D. A. | en_US |
dc.contributor.author | Santoro, M. | en_US |
dc.contributor.author | Shivers, Brenda D. | en_US |
dc.date.accessioned | 2006-09-11T14:42:47Z | |
dc.date.available | 2006-09-11T14:42:47Z | |
dc.date.issued | 1997-05 | en_US |
dc.identifier.citation | Vasilakos, J. P.; Lynch, T.; Ghayur, T.; Giegel, D. A.; Santoro, M.; Shivers, B. D.; (1997). "Caspase-3/CPP32-like activity is not sufficient to mediate apoptosis in an IL-2 dependent T cell line." Apoptosis 2(3): 289-303. <http://hdl.handle.net/2027.42/44379> | en_US |
dc.identifier.issn | 1360-8185 | en_US |
dc.identifier.issn | 1573-675X | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/44379 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=14646542&dopt=citation | en_US |
dc.description.abstract | CTLL cells undergo apoptosis when cultured in the absence of IL-2. The IL-1β-converting-enzyme (ICE)/ caspase family has been implicated as an integral component of some forms of apoptosis. Numerous members of the caspase family have been identified, and it appears as if caspase-3/CPP32 plays a critical role. Previously we demonstrated that ICE/caspase-1 expression increases in CTLL cells during apoptosis; however, inhibition of ICE activity did not abrogate apoptotic death. The purpose of this report is to determine if other members of the caspase family are involved in T cell apoptosis induced by growth factor starvation. We show that cytosolic CPP32-like activity, as measured by the cleavage of DEVD-pNA and poly(ADP-ribose) polymerase (PARP), increases during apoptosis following growth factor deprivation. Cytosolic CPP32-like activity is inhibited in cells treated with the broad spectrum ICE family inhibitor boc-aspartyl(OMe)-fluoromethylketone (D-FMK) and by VAD-FMK and DEVD-FMK which have greater specificity for CPP32-like ICE homologs; however, only the broad spectrum ICE inhibitor D-FMK inhibited apoptosis. Our results suggest that apoptosis induced by growth factor deprivation involves the caspase family, but increased CPP32-like activity is not sufficient to mediate apoptosis induced by IL-2 starvation. | en_US |
dc.format.extent | 1034496 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Kluwer Academic Publishers; Chapman and Hall ; Springer Science+Business Media | en_US |
dc.subject.other | Medicine & Public Health | en_US |
dc.subject.other | Cancer Research | en_US |
dc.subject.other | Virology | en_US |
dc.subject.other | Anatomy | en_US |
dc.subject.other | Oncology | en_US |
dc.subject.other | Biochemistry, General | en_US |
dc.subject.other | Caspase | en_US |
dc.subject.other | Fluoromethylketones | en_US |
dc.subject.other | CPP32 | en_US |
dc.subject.other | PARP | en_US |
dc.subject.other | T Cells | en_US |
dc.title | Caspase-3/CPP32-like activity is not sufficient to mediate apoptosis in an IL-2 dependent T cell line | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Radiology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Cell Biology, Parke-Davis Pharmaceutical Research Company, Division of Warner-Lambert, Ann Arbor, USA; Department of Neurology, University of Michigan, Ann Arbor, USA | en_US |
dc.contributor.affiliationum | Department of Neurology, University of Michigan, Ann Arbor, USA | en_US |
dc.contributor.affiliationum | Department of Neurology, University of Michigan, Ann Arbor, USA | en_US |
dc.contributor.affiliationother | Department of Cell Biology, Parke-Davis Pharmaceutical Research Company, Division of Warner-Lambert, Ann Arbor, USA | en_US |
dc.contributor.affiliationother | BASF Bioresearch Corporation, Worcester, USA | en_US |
dc.contributor.affiliationother | Department of Cell Biology, Parke-Davis Pharmaceutical Research Company, Division of Warner-Lambert, Ann Arbor, USA | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 14646542 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/44379/1/10495_2004_Article_172935.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1023/A:1026489104188 | en_US |
dc.identifier.source | Apoptosis | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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