Over-expression of Bcl-2 against Pteris semipinnata L-induced apoptosis of human colon cancer cells via a NF-kappa B—related pathway
dc.contributor.author | Chen, G. G. | en_US |
dc.contributor.author | Liang, N. -C. | en_US |
dc.contributor.author | Lee, J. F. Y. | en_US |
dc.contributor.author | Chan, U. P. F. | en_US |
dc.contributor.author | Wang, S. H. | en_US |
dc.contributor.author | Leung, B. C. S. | en_US |
dc.contributor.author | Leung, K. L. | en_US |
dc.date.accessioned | 2006-09-11T14:44:50Z | |
dc.date.available | 2006-09-11T14:44:50Z | |
dc.date.issued | 2004-09 | en_US |
dc.identifier.citation | Chen, G. G.; Liang, N.-C.; Lee, J. F. Y.; Chan, U. P. F.; Wang, S. H.; Leung, B. C. S.; Leung, K. L.; (2004). "Over-expression of Bcl-2 against Pteris semipinnata L-induced apoptosis of human colon cancer cells via a NF-kappa B—related pathway." APOPTOSIS 9(5): 619-627. <http://hdl.handle.net/2027.42/44400> | en_US |
dc.identifier.issn | 1360-8185 | en_US |
dc.identifier.issn | 1573-675X | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/44400 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=15314290&dopt=citation | en_US |
dc.description.abstract | Ent-11α-hydroxy-15-oxo-kaur-16-en-19-oic-acid (5F), an antitumor component, is a chemical compound isolated from Pteris semipinnata L (PsL), a Chinese traditional herb. We examined whether 5F could affect apoptosis in human colon cancer HT-29 cells, and test whether and how the over-expression of Bcl-2 and Bcl-xL could offset the effect of 5F on cell growth. The result demonstrated that 5F significantly induced apoptosis of HT-29, as shown by MTT assay and DNA fragmentation measurement. Treatment of HT-29 with 5F increased both p38 and iNOS levels, suggesting these two molecules may contribute to the apoptotic effect of 5F. Over-expression of Bcl-2 or Bcl-xL attenuated the increase of p38 and iNOS induced by 5F. The cells with Bcl-2 or Bcl-xL over-expression showed an elevation of nuclear factor kappa B (NF-kappa B) activity, accompanying a significant reduction of 5F-induced apoptosis. Furthermore, inhibition of NF-kappa B by I k BαSR, which is a powerful inhibitor of NF-kappa B, restored the ability of 5F to induce apoptosis in the cells transfected with Bcl-2. These data strongly indicated that the apoptotic effect of 5F on HT-29 was closely associated with the activity of NF-kappa B, which was up-regulated by Bcl-2 and Bcl-xL. In conclusion, 5F induced apoptosis in HT-29 cells and this apoptotic effect was associated with the high level of p38 and iNOS expression. The apoptotic effect of 5F could be significantly offset by over-expression of either Bcl-2 or Bcl-xL. Bcl-2, and to the less extent, Bcl-xL, were able to increase the activity of NF-kappa B, which was a known anti-apoptotic molecule in human colon cancer cells. | en_US |
dc.format.extent | 336409 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Kluwer Academic Publishers; Springer Science+Business Media | en_US |
dc.subject.other | Medicine & Public Health | en_US |
dc.subject.other | Cancer Research | en_US |
dc.subject.other | Virology | en_US |
dc.subject.other | Anatomy | en_US |
dc.subject.other | Oncology | en_US |
dc.subject.other | Biochemistry, General | en_US |
dc.subject.other | Apoptosis | en_US |
dc.subject.other | Bcl-2 | en_US |
dc.subject.other | Colon Cancer | en_US |
dc.subject.other | Nuclear Factor Kappa B | en_US |
dc.subject.other | Pteris Semipinnata | en_US |
dc.title | Over-expression of Bcl-2 against Pteris semipinnata L-induced apoptosis of human colon cancer cells via a NF-kappa B—related pathway | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Radiology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Internal Medicine, Medical School, University of Michigan, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationother | Department of Surgery, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, NT, Hong Kong | en_US |
dc.contributor.affiliationother | Institute of Biochemistry and Molecular Biology, Guangdong Medical College, Zhanjiang, Guangdong, People's Republic of China | en_US |
dc.contributor.affiliationother | Department of Surgery, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, NT, Hong Kong | en_US |
dc.contributor.affiliationother | Department of Surgery, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, NT, Hong Kong | en_US |
dc.contributor.affiliationother | Department of Surgery, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, NT, Hong Kong | en_US |
dc.contributor.affiliationother | Department of Surgery, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, NT, Hong Kong | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 15314290 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/44400/1/10495_2004_Article_5277461.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1023/B:APPT.0000038041.57782.84 | en_US |
dc.identifier.source | APOPTOSIS | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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