Use of Flow Cytometry to Quantify Mouse Gastric Epithelial Cell Populations
dc.contributor.author | Zavros, Yana | en_US |
dc.contributor.author | Van Antwerp, Mary E. | en_US |
dc.contributor.author | Merchant, Juanita L. | en_US |
dc.date.accessioned | 2006-09-11T14:47:09Z | |
dc.date.available | 2006-09-11T14:47:09Z | |
dc.date.issued | 2000-06 | en_US |
dc.identifier.citation | Zavros, Yana; Van Antwerp, Mary; Merchant, Juanita L.; (2000). "Use of Flow Cytometry to Quantify Mouse Gastric Epithelial Cell Populations." Digestive Diseases and Sciences 45(6): 1192-1199. <http://hdl.handle.net/2027.42/44428> | en_US |
dc.identifier.issn | 0163-2116 | en_US |
dc.identifier.issn | 1573-2568 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/44428 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=10877237&dopt=citation | en_US |
dc.description.abstract | Flow cytometry provides the opportunity to quantify cell populations within a total cell suspension. The quality of flow cytometry is strongly dependent on the isolation of intact viable cells. However, techniques to isolate mouse gastric cells for flow cytometry have not been evaluated. The objective of this study was to develop an effective method for isolating intact viable cells from mouse gastric tissue for flow cytometry. Cells were isolated from mouse stomach and spleen by either enzymatic separation or mechanical dissociation. A Percoll density gradient was used to separate viable cells from cellular debris. Cells were labeled with fluorescently tagged ligand or antibody and analyzed by flow cytometry. According to propidium iodide staining, there was a higher percentage of viable cells after mechanical dissociation (10–20%) compared to enzymatic separation (1%). After Percoll centrifugation there was a further increase in the percent of viable cells (50–80%). Gastrin (G), somatostatin (D), and parietal cells represented 0.6%, 3%, and 8% of the total epithelial cell population, respectively. T and B lymphocytes made up 4% and 2% in the gastric mucosa. Dissociated splenocytes were comprised of 20% T cells and 14% B cells. The ability to reliably resolve a cellular fraction that comprises only 0.6% of the input marks a substantial improvement over morphometric methods. Therefore, mechanical dissociation of the stomach followed by use of a Percoll gradient is the preferred method for isolating viable intact gastric epithelial cells for flow cytometry. | en_US |
dc.format.extent | 104881 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Kluwer Academic Publishers-Plenum Publishers; Plenum Publishing Corporation ; Springer Science+Business Media | en_US |
dc.subject.other | Parietal Cells | en_US |
dc.subject.other | Gastrin | en_US |
dc.subject.other | Helicobacter Pylori | en_US |
dc.subject.other | Hepatology | en_US |
dc.subject.other | Medicine & Public Health | en_US |
dc.subject.other | Gastroenterology | en_US |
dc.subject.other | Oncology | en_US |
dc.subject.other | Transplant Surgery | en_US |
dc.subject.other | Biochemistry, General | en_US |
dc.subject.other | Gastric Epithelial Cells | en_US |
dc.subject.other | Somatostatin | en_US |
dc.title | Use of Flow Cytometry to Quantify Mouse Gastric Epithelial Cell Populations | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Internal Medicine and Specialties | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Howard Hughes Medical Institute, and the Departments of Internal Medicine and Physiology, University of Michigan, Ann Arbor, Michigan, 48109 | en_US |
dc.contributor.affiliationum | Howard Hughes Medical Institute, and the Departments of Internal Medicine and Physiology, University of Michigan, Ann Arbor, Michigan, 48109 | en_US |
dc.contributor.affiliationum | Howard Hughes Medical Institute, and the Departments of Internal Medicine and Physiology, University of Michigan, Ann Arbor, Michigan, 48109 | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 10877237 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/44428/1/10620_2004_Article_224654.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1023/A:1005514422187 | en_US |
dc.identifier.source | Digestive Diseases and Sciences | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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