Use of Air-Liquid Two-Phase Flow in Hydrophobic Microfluidic Channels for Disposable Flow Cytometers
dc.contributor.author | Huh, Dongeun | en_US |
dc.contributor.author | Tung, Yi-Chung | en_US |
dc.contributor.author | Wei, Hsien-Hung | en_US |
dc.contributor.author | Grotberg, James B. | en_US |
dc.contributor.author | Skerlos, Steven J. | en_US |
dc.contributor.author | Kurabayashi, Katsuo | en_US |
dc.contributor.author | Takayama, Shuichi | en_US |
dc.date.accessioned | 2006-09-11T14:50:53Z | |
dc.date.available | 2006-09-11T14:50:53Z | |
dc.date.issued | 2002-05 | en_US |
dc.identifier.citation | Huh, Dongeun; Tung, Yi-Chung; Wei, Hsien-Hung; Grotberg, James B.; Skerlos, Steven J.; Kurabayashi, Katsuo; Takayama, Shuichi; (2002). "Use of Air-Liquid Two-Phase Flow in Hydrophobic Microfluidic Channels for Disposable Flow Cytometers." Biomedical Microdevices 4(2): 141-149. <http://hdl.handle.net/2027.42/44472> | en_US |
dc.identifier.issn | 1387-2176 | en_US |
dc.identifier.issn | 1572-8781 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/44472 | |
dc.description.abstract | This paper describes a disposable flow cytometer that uses an air-liquid two-phase microfluidic system to produce a focused high-speed liquid sample stream of particles and cells. The susceptibility of thin liquid columns to instabilities may suggest that focusing of sample liquids with streams of air would be difficult. The design of channel geometry, control of flow rates, and use of appropriate surface chemistries on the channel walls, however, enabled the generation of thin (15–100 μm) and partially bounded sample streams that were stable and suitable for rapid cell analysis. Using an inverted epi-fluorescence microscope with a photo-multiplier tube, we demonstrated that the system is capable of counting the number of beads and C 2 C 12 myoblast cells. The effects of different flow rates and surface chemistries of the channel walls on the air-liquid two-phase flows were characterized using optical and confocal microscopy. Use of air instead of liquids as a sheath fluid eliminates the need for large sheath liquid reservoirs, and reduces the volume and weight requirements. The low manufacturing cost and high volumetric efficiency make the air-sheath flow cytometer attractive for use as a stand-alone device or as an integrated component of bio-artificial hybrid microsystems. | en_US |
dc.format.extent | 1046317 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Kluwer Academic Publishers; Springer Science+Business Media | en_US |
dc.subject.other | Engineering | en_US |
dc.subject.other | Characterization and Evaluation Materials | en_US |
dc.subject.other | Pharmacy | en_US |
dc.subject.other | Biophysics/Biomedical Physics | en_US |
dc.subject.other | Electronic and Computer Engineering | en_US |
dc.subject.other | Biomedical Engineering | en_US |
dc.subject.other | Air Sheath | en_US |
dc.subject.other | Flow Cytometer | en_US |
dc.subject.other | Two-phase Flow | en_US |
dc.subject.other | Surface Chemistry | en_US |
dc.subject.other | Cell Counting | en_US |
dc.title | Use of Air-Liquid Two-Phase Flow in Hydrophobic Microfluidic Channels for Disposable Flow Cytometers | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbsecondlevel | Biomedical Engineering | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Engineering | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biomedical Engineering, University of Michigan, Ann Arbor, 3304; GG Brown, 2350 Hayward, Ann Arbor, MI, 48109-2125 | en_US |
dc.contributor.affiliationum | Department of Mechanical Engineering, University of Michigan, Ann Arbor, 2272; GG Brown, 2350 Hayward, Ann Arbor, MI, 48109-2125 | en_US |
dc.contributor.affiliationum | Department of Biomedical Engineering, University of Michigan, Ann Arbor, 3304; GG Brown, 2350 Hayward, Ann Arbor, MI, 48109-2125 | en_US |
dc.contributor.affiliationum | Department of Biomedical Engineering, University of Michigan, Ann Arbor, 3304; GG Brown, 2350 Hayward, Ann Arbor, MI, 48109-2125 | en_US |
dc.contributor.affiliationum | Department of Mechanical Engineering, University of Michigan, Ann Arbor, 2272; GG Brown, 2350 Hayward, Ann Arbor, MI, 48109-2125 | en_US |
dc.contributor.affiliationum | Department of Mechanical Engineering, University of Michigan, Ann Arbor, 2272; Department of Macromolecular Science and Engineering, University of Michigan, Ann Arbor, Ann Arbor, MI, 48109-2125; GG Brown, 2350 Hayward, Ann Arbor, MI, 48109-2125 | en_US |
dc.contributor.affiliationum | Department of Biomedical Engineering, University of Michigan, Ann Arbor, 3304; Department of Macromolecular Science and Engineering, University of Michigan, Ann Arbor, Ann Arbor, MI, 48109-2125; GG Brown, 2350 Hayward, Ann Arbor, MI, 48109-2125 | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/44472/1/10544_2004_Article_398974.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1023/A:1014691416614 | en_US |
dc.identifier.source | Biomedical Microdevices | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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