Effects of interleukin-1 β and tumor necrosis factor-α on osteoblastic expression of osteocalcin and mineralized extracellular matrix in vitro
dc.contributor.author | Hauschka, Peter V. | en_US |
dc.contributor.author | Taichman, Russell S. | en_US |
dc.date.accessioned | 2006-09-11T14:54:34Z | |
dc.date.available | 2006-09-11T14:54:34Z | |
dc.date.issued | 1992-12 | en_US |
dc.identifier.citation | Taichman, Russell S.; Hauschka, Peter V.; (1992). "Effects of interleukin-1 β and tumor necrosis factor-α on osteoblastic expression of osteocalcin and mineralized extracellular matrix in vitro." Inflammation 16(6): 587-601. <http://hdl.handle.net/2027.42/44508> | en_US |
dc.identifier.issn | 0360-3997 | en_US |
dc.identifier.issn | 1573-2576 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/44508 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=1459694&dopt=citation | en_US |
dc.description.abstract | Osteoblasts play a pivotal role during the bioresponse of bone to agents that stimulate bone resorption and/or inhibit bone formation including hormones, polypeptide growth factors, and cytokines. We examined the cytokines interleukin-1-beta (IL-1 β ) and tumor necrosis factor-alpha (TNF- α ) for their effects on osteoblastic proliferation and development and expression of alkaline phosphatase and the osteoblast-specific protein osteocalcin in a mineralizing environment. Primary rat osteoblast-like cells (ROB) and osteoblastic cell lines derived from rat (ROS 17/2.8) and human (MG-63) osteosarcomas were studied. IL-1 β and TNF- α were chosen because of their critical importance during the host response to local inflammatory stimuli. Qualitatively similar two- to threefold inhibition of osteocalcin synthesis by IL-1β and TNF- α were observed in all three postconfluent bone-forming model systems. Because of the readily measurable concentrations of osteocalcin produced in our culture protocol, it was not necessary to enhance osteoblastic synthesis of osteocalcin by supplementation with 1,25(OH) 2 -vitamin D 3 , a treatment which exerts pleiotropic effects on osteoblasts. Under the constraints of our protocol, where alkaline phosphatase and mineralization were already elevated at the 14-day onset of treatment, neither of these phenotypic properties was sensitive to a three-day cytokine exposure. Differences were noted in proliferation, where only TNF- α stimulated DNA synthesis in ROB cells, while both cytokines stimulated MG-63 cells. IL-1 β and TNF- α failed to alter ROS 17/2.8 DNA synthesis except at the highest doses (25 pM IL-1β and l nM TNF- α ) where inhibition was observed. These results further support the view that cytokine-mediated osteoblastic regulation can be relatively selective. | en_US |
dc.format.extent | 914433 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Kluwer Academic Publishers-Plenum Publishers; Plenum Publishing Corporation ; Springer Science+Business Media | en_US |
dc.subject.other | Medicine & Public Health | en_US |
dc.subject.other | Rheumatology | en_US |
dc.subject.other | Internal Medicine | en_US |
dc.subject.other | Pharmacology/Toxicology | en_US |
dc.subject.other | Pathology | en_US |
dc.title | Effects of interleukin-1 β and tumor necrosis factor-α on osteoblastic expression of osteocalcin and mineralized extracellular matrix in vitro | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Pathology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Periodontology, Harvard School of Dental Medicine, 188 Longwood Avenue, 02115, Boston, MA; Department of Orthopaedic Surgery, The Children's Hospital Corporation, Enders-12, 300 Longwood Avenue, 02115, Boston, Massachusetts; Department of Periodontics, Prevention and Geriatrics, School of Dentistry, University of Michigan, 1011 North University Ave., 48109-1078, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationother | Department of Orthopaedic Surgery, The Children's Hospital Corporation, Enders-12, 300 Longwood Avenue, 02115, Boston, Massachusetts; Department of Oral Biology, Harvard School of Dental Medicine, 188 Longwood Avenue, 02115, Boston, MA | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 1459694 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/44508/1/10753_2004_Article_BF00919342.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/BF00919342 | en_US |
dc.identifier.source | Inflammation | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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