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Neutrophil Chemotactic Activity and C5a Following Systemic Activation of Complement in Rats
dc.contributor.authorSchmid, Elisabethen_US
dc.contributor.authorWarner, Roscoe L.en_US
dc.contributor.authorCrouch, Larry D.en_US
dc.contributor.authorFriedl, Hans P.en_US
dc.contributor.authorTill, Gerd O.en_US
dc.contributor.authorHugli, Tony E.en_US
dc.contributor.authorWard, Peter A.en_US
dc.date.accessioned2006-09-11T14:55:18Z
dc.date.available2006-09-11T14:55:18Z
dc.date.issued1997-06en_US
dc.identifier.citationSchmid, Elisabeth; Warner, Roscoe L.; Crouch, Larry D.; Friedl, Hans P.; Till, Gerd O.; Hugli, Tony E.; Ward, Peter A.; (1997). "Neutrophil Chemotactic Activity and C5a Following Systemic Activation of Complement in Rats." Inflammation 21(3): 325-333. <http://hdl.handle.net/2027.42/44517>en_US
dc.identifier.issn0360-3997en_US
dc.identifier.issn1573-2576en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/44517
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=9246574&dopt=citationen_US
dc.description.abstractUsing ELISA analysis, rat C5a was stimulated in serum from rats undergoing systemic activation of complement after intravenous infusion of purified cobra venom factor (CVF). Biological (neutrophil chemotactic) activity was also assessed. Serum levels of C5a were directly proportional to the amount of CVF infused. C5a and neutrophil chemotactic activity, peaked by 5 min, then plateaued. In vitro addition of anti-C5a to serum samples of CVF-infused rats totally abolished chemotactic activity, indicating that all biological activity could be ascribed to C5a. Blood neutrophils obtained from CVF-infused animals showed a significant upregulation of CD11b, the increase being reduced (38%) in animals pretreated with anti-C5a. These findings indicate that infusion of CVF into rats produces generation of C5a, all chemotactic activity in serum being related to C5a. The in vivo generation of C5a is, at least inpart, responsible for upregulation of CD11b on blood neutrophils.en_US
dc.format.extent491737 bytes
dc.format.extent3115 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherKluwer Academic Publishers-Plenum Publishers; Plenum Publishing Corporation ; Springer Science+Business Mediaen_US
dc.subject.otherMedicine & Public Healthen_US
dc.subject.otherRheumatologyen_US
dc.subject.otherPharmacology/Toxicologyen_US
dc.subject.otherInternal Medicineen_US
dc.subject.otherPathologyen_US
dc.titleNeutrophil Chemotactic Activity and C5a Following Systemic Activation of Complement in Ratsen_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelPathologyen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Pathology, The University of Michigan Medical School, Ann Arbor, Michigan, 48109en_US
dc.contributor.affiliationumDepartment of Pathology, The University of Michigan Medical School, Ann Arbor, Michigan, 48109en_US
dc.contributor.affiliationumDepartment of Pathology, The University of Michigan Medical School, Ann Arbor, Michigan, 48109en_US
dc.contributor.affiliationumDepartment of Pathology, The University of Michigan Medical School, Ann Arbor, Michigan, 48109; Department of Surgery, The University of Zürich Medical School, Zürich, Switzerlanden_US
dc.contributor.affiliationumDepartment of Pathology, The University of Michigan Medical School, Ann Arbor, Michigan, 48109en_US
dc.contributor.affiliationotherDepartment of Immunology, Scripps Research Institute, La Jolla, California, 92037en_US
dc.contributor.affiliationotherDepartment of Surgery, The University of Zürich Medical School, Zürich, Switzerlanden_US
dc.contributor.affiliationumcampusAnn Arboren_US
dc.identifier.pmid9246574en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/44517/1/10753_2004_Article_426059.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1023/A:1027302017117en_US
dc.identifier.sourceInflammationen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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