Loxosceles Spider Venom Induces the Production of α and β Chemokines: Implications for the Pathogenesis of Dermonecrotic Arachnidism
dc.contributor.author | Miller, Mark J. | en_US |
dc.contributor.author | Gomez, Hernan F. | en_US |
dc.contributor.author | Desai, Anjali | en_US |
dc.contributor.author | Warren, Jeffrey S. | en_US |
dc.date.accessioned | 2006-09-11T14:55:53Z | |
dc.date.available | 2006-09-11T14:55:53Z | |
dc.date.issued | 1999-06 | en_US |
dc.identifier.citation | Gomez, Hernan F.; Miller, Mark J.; Desai, Anjali; Warren, Jeffrey S.; (1999). " Loxosceles Spider Venom Induces the Production of α and β Chemokines: Implications for the Pathogenesis of Dermonecrotic Arachnidism." Inflammation 23(3): 207-215. <http://hdl.handle.net/2027.42/44524> | en_US |
dc.identifier.issn | 0360-3997 | en_US |
dc.identifier.issn | 1573-2576 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/44524 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=10392755&dopt=citation | en_US |
dc.description.abstract | Bites from the brown recluse spider and other Loxosceles arachnids result in dermonecrotic skin lesions. Neutrophils (PMN) are essential to the development of Loxosceles -induced skin lesions, but paradoxically, in vitro PMN activation is inhibited by direct exposure to Loxosceles venom. Neutrophil activation occurs in response to a myriad of soluble mediators that include members of both the α and β chemokine families. Because arachnid envenomation results in the exposure of several different cell types to venom, we investigated venom-induced expression of α and β chemokines in both endothelial cells (human umbilical vein; HUVEC) and epithelial cells (A549 pneumocytes). Chemokine-specific capture enzyme immunoassays (EIA) were used to measure Loxosceles deserta venom-induced α chemokines: interleukin-8 (IL-8), growth-related oncogene-alpha (GRO-α), and β chemokines: monocyte chemoattractant protein-1 (MCP-1), and regulated on activation, normal T cell expressed and secreted (RANTES) in cell-free conditioned media from HUVEC and A549 cell monolayers. Exposure of HUVECs (8 h) to Loxosceles venom resulted in the production of IL-8 (5.2 ± 1.30 ng/ml), MCP-1 (1.44 ± 0.11 ng/ml) and GRO-α (1.97 ± 0.15 ng/ml) in a dose and time-dependent manner. Exposure of A549 cell monolayers to venom resulted in IL-8 (7.74 ± 0.30 ng/ml), and MCP-1 (2.61 ± 0.31 ng/ml), but neither GRO-α nor RANTES accumulated during an 8-hour incubation period. Chemokines accumulated in a venom dose and time-dependent manner. Neither cell type secreted RANTES in response to Loxosceles venom. These data indicate that Loxosceles spider venom is a potent inducer of α and β chemokines in both endothelial and epithelial cell types. Based on the established roles of IL-8, MCP-1, and GRO-α, in inflammation, these observations have relevance to the pathophysiology of Loxosceles -Induced dermonecrosis. | en_US |
dc.format.extent | 423598 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Kluwer Academic Publishers-Plenum Publishers; Plenum Publishing Corporation ; Springer Science+Business Media | en_US |
dc.subject.other | Rheumatology | en_US |
dc.subject.other | Medicine & Public Health | en_US |
dc.subject.other | Pharmacology/Toxicology | en_US |
dc.subject.other | Internal Medicine | en_US |
dc.subject.other | Pathology | en_US |
dc.title | Loxosceles Spider Venom Induces the Production of α and β Chemokines: Implications for the Pathogenesis of Dermonecrotic Arachnidism | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Pathology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Pathology, University of Michigan Medical Center, USA | en_US |
dc.contributor.affiliationum | Department of Pathology, University of Michigan Medical Center, USA | en_US |
dc.contributor.affiliationum | Department of Pathology, University of Michigan Medical Center, USA | en_US |
dc.contributor.affiliationother | Department of Surgery, Section of Emergency Medicine, USA | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 10392755 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/44524/1/10753_2004_Article_408823.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1023/A:1020217818245 | en_US |
dc.identifier.source | Inflammation | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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