Tannin assays in ecological studies Precipitation of ribulose-1,5-bisphosphate carboxylase/oxygenase by tannic acid, quebracho, and oak foliage extracts
dc.contributor.author | Martin, Joan Stadler | en_US |
dc.contributor.author | Martin, Michael M. | en_US |
dc.date.accessioned | 2006-09-11T15:25:26Z | |
dc.date.available | 2006-09-11T15:25:26Z | |
dc.date.issued | 1983-02 | en_US |
dc.identifier.citation | Martin, Joan Stadler; Martin, Michael M.; (1983). "Tannin assays in ecological studies Precipitation of ribulose-1,5-bisphosphate carboxylase/oxygenase by tannic acid, quebracho, and oak foliage extracts." Journal of Chemical Ecology 9(2): 285-294. <http://hdl.handle.net/2027.42/44903> | en_US |
dc.identifier.issn | 1573-1561 | en_US |
dc.identifier.issn | 0098-0331 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/44903 | |
dc.description.abstract | Tannic acid and quebracho precipitate many times their weight of the abundant leaf protein, ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBPC). The use of this protein in protein precipitation assays for tannin content is described. Extracts of mature foliage of pin, bur, and black oak precipitate 2.01, 0.69, and 0.09 mg RuBPC/ mg (dry wt) of leaf powder extracted, respectively, at pH 6.1. From these measurements it can be calculated that all three of these oak species have sufficient tannins to precipitate all of the RuBPC present in their foliage. At mildly alkaline pHs, however (pH ⩾ 7.5), RuBPC is not precipitated by tannins. Since RuBPCis the most abundant protein present in photosynthetic tissues, often constituting as much as 50% of the soluble proteins and 25% of the total proteins in leaf tissue, the interactions of this protein with tannins are highly relevant to an evaluation of the role of tannins as antiherbivore, digestibility-reducing substances. Our measurements provide no basis for arguing that differences in tannin levels in different species reflect differences in the digestibility of leaf proteins or that tannins have any effect whatsoever upon the digestibility of leaf protein under conditions which normally prevail in most insects' guts. These findings emphasize the need to test more of the assumptions underlying contemporary interpretations of the importance of tannins in plant herbivore interactions. | en_US |
dc.format.extent | 606663 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Kluwer Academic Publishers-Plenum Publishers; Plenum Publishing Corporation ; Springer Science+Business Media | en_US |
dc.subject.other | Herbivory | en_US |
dc.subject.other | Ecology | en_US |
dc.subject.other | Physical Chemistry | en_US |
dc.subject.other | Quercus | en_US |
dc.subject.other | Allelochemics | en_US |
dc.subject.other | Life Sciences | en_US |
dc.subject.other | Agriculture | en_US |
dc.subject.other | Biological Microscopy | en_US |
dc.subject.other | Chemical Defense | en_US |
dc.subject.other | Tannins | en_US |
dc.subject.other | Digestibility Reducing Substances | en_US |
dc.subject.other | RuBPC | en_US |
dc.title | Tannin assays in ecological studies Precipitation of ribulose-1,5-bisphosphate carboxylase/oxygenase by tannic acid, quebracho, and oak foliage extracts | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Division of Biological Sciences, University of Michigan, 48109, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Division of Biological Sciences, University of Michigan, 48109, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 24407347 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/44903/1/10886_2004_Article_BF00988046.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/BF00988046 | en_US |
dc.identifier.source | Journal of Chemical Ecology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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