Stability of the contractile assembly and Ca 2+ - activated tension in adenovirus infected adult cardiac myocytes
dc.contributor.author | Westfall, Margaret V. | en_US |
dc.contributor.author | Rust, Elizabeth M. | en_US |
dc.contributor.author | Metzger, Joseph M. | en_US |
dc.date.accessioned | 2006-09-11T15:56:22Z | |
dc.date.available | 2006-09-11T15:56:22Z | |
dc.date.issued | 1998-04 | en_US |
dc.identifier.citation | Rust, Elizabeth M.; Westfall, Margaret V.; Metzger, Joseph M.; (1998). "Stability of the contractile assembly and Ca 2+ - activated tension in adenovirus infected adult cardiac myocytes." Molecular and Cellular Biochemistry 181 (1-2): 143-155. <http://hdl.handle.net/2027.42/45333> | en_US |
dc.identifier.issn | 1573-4919 | en_US |
dc.identifier.issn | 0300-8177 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/45333 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=9562251&dopt=citation | en_US |
dc.description.abstract | Adenovirus-mediated gene transfer into adult cardiac myocytes in primary culture is a potentially useful method to study the structure and function of the contractile apparatus. However, the consequences of adenovirus infection on the highly differentiated state of the cultured myocyte have not been determined. We report here a detailed analysis of myofilament structure and function over time in primary culture and after adenovirus infection. Adult rat ventricular myocytes in primary culture were infected with a recombinant adenovirus vector expressing either the LacZ or alkaline phosphatase reporter gene. Control and infected myocytes were collected at days 0-7 post-isolation/infection, and myofilament isoform expression was determined by SDS-PAGE and Western blot. Laser scanning densitometry showed that the α- to β-myosin heavy chain ratio, the stoichiometry of the myosin light chains and the expression of the adult troponin T isoform did not change over time in culture or with adenovinus treatment. Importantly, examination of Ca 2+ -activated tension in single myocytes showed no change in the shape or position of the tension-pCa relationship in the control and adenovirus infected myocytes during primary culture. These results indicate that the structure and function of adult cardiac myocytes are stable in short term primary culture and are not affected by adenovirus infection per se, and therefore provide the foundation for the use of adenovirus-mediated myofilament gene transfer to study contractile apparatus structure and function in adult cardiac myocytes.ain. | en_US |
dc.format.extent | 337375 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Kluwer Academic Publishers; Springer Science+Business Media | en_US |
dc.subject.other | Oncology | en_US |
dc.subject.other | Cardiac Muscle | en_US |
dc.subject.other | Cardiology | en_US |
dc.subject.other | Life Sciences | en_US |
dc.subject.other | Medical Biochemistry | en_US |
dc.subject.other | Biochemistry, General | en_US |
dc.subject.other | Contractility | en_US |
dc.subject.other | Myofilament | en_US |
dc.subject.other | Virus | en_US |
dc.title | Stability of the contractile assembly and Ca 2+ - activated tension in adenovirus infected adult cardiac myocytes | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Physiology, School of Medicine, University of Michigan, Ann Arbor, MI, 48109-0622, USA | en_US |
dc.contributor.affiliationum | Department of Physiology, School of Medicine, University of Michigan, Ann Arbor, MI, 48109-0622, USA | en_US |
dc.contributor.affiliationum | Department of Physiology, School of Medicine, University of Michigan, Ann Arbor, MI, 48109-0622, USA | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 9562251 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/45333/1/11010_2004_Article_152664.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1023/A:1006802719136 | en_US |
dc.identifier.source | Molecular and Cellular Biochemistry | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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