Cell line dependent involvement of ceramide in ultraviolet light-induced apoptosis
dc.contributor.author | Chatterjee, Madhumita | en_US |
dc.contributor.author | Wu, Shiyong | en_US |
dc.date.accessioned | 2006-09-11T15:56:38Z | |
dc.date.available | 2006-09-11T15:56:38Z | |
dc.date.issued | 2001-03 | en_US |
dc.identifier.citation | Chatterjee, Madhumita; Wu, Shiyong; (2001). "Cell line dependent involvement of ceramide in ultraviolet light-induced apoptosis." Molecular and Cellular Biochemistry 219 (1-2): 21-27. <http://hdl.handle.net/2027.42/45337> | en_US |
dc.identifier.issn | 0300-8177 | en_US |
dc.identifier.issn | 1573-4919 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/45337 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=11354249&dopt=citation | en_US |
dc.description.abstract | Ultraviolet light (UV) activates an acid sphingomyelinase (ASMase) pathway, which hydrolyzes sphingomyeline to ceramide. Ceramide has been found to be a second messenger, which activates the c-jun N-terminal kinase (JNK) that is required for apoptotic cell death. However, the role of ceramide in UV-induced JNK activation and apoptosis remains controversial. In this study, we examined the correlation among ceramide production, JNK activation and cell apoptosis after UV-irradiation in three cell lines: 293 (kidney), Jurkat (lymphocytes) and MCF-7 (breast) were used in this study. The ceramide production was analyzed using the diacylglycerol kinase assay method. The JNK activation was measured by Western blot analysis using an antibody specifically recognizing phosphorylated JNK. Cell apoptosis was determined by morphological change or flow cytometry. Our data show that UV-irradiation induces ceramide production in both 293 and Jurkat cells. Inhibition of ceramide production by desipramine (25–50 μM) reduced UV-induced JNK activation in both 293 and Jurkat cells; and protects 293 cells from UV-induced apoptosis. However, inhibition of ceramide production does not prevent Jurkat cells from UV-induced apoptosis. In addition, our data demonstrates that UV-irradiation induces JNK activation and apoptosis of MCF-7 cells without production of detectable amounts of ceramide after UV-irradiation. These results suggest that UV-induced JNK activation and apoptosis can be mediated through a ceramide dependent or an independent pathway. | en_US |
dc.format.extent | 570229 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Kluwer Academic Publishers; Springer Science+Business Media | en_US |
dc.subject.other | Ultraviolet Light | en_US |
dc.subject.other | Cardiology | en_US |
dc.subject.other | Life Sciences | en_US |
dc.subject.other | Medical Biochemistry | en_US |
dc.subject.other | Oncology | en_US |
dc.subject.other | Biochemistry, General | en_US |
dc.subject.other | Ceramide | en_US |
dc.subject.other | Apoptosis | en_US |
dc.subject.other | Sphingomyelinase | en_US |
dc.title | Cell line dependent involvement of ceramide in ultraviolet light-induced apoptosis | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Radiation Oncology, Division of Radiation and Cancer Biology, University of Michigan Medical School, Ann Arbor, MI, 48109, USA | en_US |
dc.contributor.affiliationum | Department of Radiation Oncology, Division of Radiation and Cancer Biology, University of Michigan Medical School, Ann Arbor, MI, 48109, USA | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 11354249 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/45337/1/11010_2004_Article_323172.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1023/A:1011083818452 | en_US |
dc.identifier.source | Molecular and Cellular Biochemistry | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.