Hormonal regulation of plasminogen activator in rat hepatoma cells
dc.contributor.author | Coleman, Patrick L. | en_US |
dc.contributor.author | Cwikel, Bernard J. | en_US |
dc.contributor.author | Barouski-Miller, Patricia A. | en_US |
dc.contributor.author | Gelehrter, Thomas D. | en_US |
dc.date.accessioned | 2006-09-11T15:57:14Z | |
dc.date.available | 2006-09-11T15:57:14Z | |
dc.date.issued | 1983-03 | en_US |
dc.identifier.citation | Gelehrter, Thomas D.; Barouski-Miller, Patricia A.; Coleman, Patrick L.; Cwikel, Bernard J.; (1983). "Hormonal regulation of plasminogen activator in rat hepatoma cells." Molecular and Cellular Biochemistry 53-54 (1-2): 11-21. <http://hdl.handle.net/2027.42/45346> | en_US |
dc.identifier.issn | 0300-8177 | en_US |
dc.identifier.issn | 1573-4919 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/45346 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=6312282&dopt=citation | en_US |
dc.description.abstract | Plasminogen activators are membrane-associated, arginine-specific serine proteases which convert the inactive plasma zymogen plasminogen to plasmin, an active, broad-spectrum serine protease. Plasmin, the major fibrinolytic enzyme in blood, also participates in a number of physiologic functions involving protein processing and tissue remodelling, and may play an important role in tumor invasion and metastasis. In HTC rat hepatoma cells in. tissue culture, glucocorticoids rapidly decrease plasminogen activator (PA) activity. We have shown that this decrease is mediated by induction of a soluble inhibitor of PA activity rather than modulation of the amount of PA. The hormonally-induced inhibitor is a cellular product which specifically inhibits PA but not plasmin. We have isolated variant lines of HTC cells which are selectively resistant to the glucocorticoid inhibition of PA but retain other glucocorticoid responses. These variants lack the hormonally-induced inhibitor; PA from these variants is fully sensitive to inhibition by inhibitor from steroid-treated wild-type cells. Cyclic nucleotides dramatically stimulate PA activity in HTC cells in a time- and concentration-dependent manner. Paradoxically, glucocorticoids further enhance this stimulation. Thus glucocorticoids exert two separate and opposite effects on PA activity. The availability of glucocorticoid-resistant variant cell lines, together with the unique regulatory interactions of steroids and cyclic nucleotides, make HTC cells a useful experimental system in which to study the multihormonal regulation of plasminogen activator. | en_US |
dc.format.extent | 830401 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Kluwer Academic Publishers; Martinus Nijhoff Publishers ; Springer Science+Business Media | en_US |
dc.subject.other | Oncology | en_US |
dc.subject.other | Cardiology | en_US |
dc.subject.other | Life Sciences | en_US |
dc.subject.other | Medical Biochemistry | en_US |
dc.subject.other | Biochemistry, General | en_US |
dc.title | Hormonal regulation of plasminogen activator in rat hepatoma cells | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Departments of Internal Medicine and Human Genetics, University of Michigan Medical School, 48109, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationum | Departments of Internal Medicine and Human Genetics, University of Michigan Medical School, 48109, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationum | Departments of Internal Medicine and Human Genetics, University of Michigan Medical School, 48109, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationum | Departments of Internal Medicine and Human Genetics, University of Michigan Medical School, 48109, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 6312282 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/45346/1/11010_2004_Article_BF00225243.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/BF00225243 | en_US |
dc.identifier.source | Molecular and Cellular Biochemistry | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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