Effect of carbon starvation on toluene degradation activity by toluene monooxygenase-expressing bacteria
dc.contributor.author | Johnson, David R. | en_US |
dc.contributor.author | Park, Joonhong | en_US |
dc.contributor.author | Kukor, Jerome J. | en_US |
dc.contributor.author | Abriola, Linda M. | en_US |
dc.date.accessioned | 2006-09-11T15:57:42Z | |
dc.date.available | 2006-09-11T15:57:42Z | |
dc.date.issued | 2006-02-14 | en_US |
dc.identifier.citation | Johnson, David R.; Park, Joonhong; Kukor, Jerome J.; Abriola, Linda M.; (2006 ). "Effect of carbon starvation on toluene degradation activity by toluene monooxygenase-expressing bacteria." Biodegradation ( ): 1-9. <http://hdl.handle.net/2027.42/45353> | en_US |
dc.identifier.issn | 0923-9820 | en_US |
dc.identifier.issn | 1572-9729 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/45353 | |
dc.description.abstract | Subsurface bacteria commonly exist in a starvation state with only periodic exposure to utilizable sources of carbon and energy. In this study, the effect of carbon starvation on aerobic toluene degradation was quantitatively evaluated with a selection of bacteria representing all the known toluene oxygenase enzyme pathways. For all the investigated strains, the rate of toluene biodegradation decreased exponentially with starvation time. First-order deactivation rate constants for TMO-expressing bacteria were approximately an order of magnitude greater than those for other oxygenase-expressing bacteria. When growth conditions (the type of growth substrate and the type and concentration of toluene oxygenase inducer) were varied in the cultures prior to the deactivation experiments, the rate of deactivation was not significantly affected, suggesting that the rate of deactivation is independent of previous substrate/inducer conditions. Because TMO-expressing bacteria are known to efficiently detoxify TCE in subsurface environments, these findings have significant implications for in situ TCE bioremediation, specifically for environments experiencing variable growth-substrate exposure conditions. | en_US |
dc.format.extent | 247909 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Kluwer Academic Publishers; Springer Science+Business Medaia Inc. | en_US |
dc.subject.other | Aromatic Oxygenase | en_US |
dc.subject.other | Carbon Starvation | en_US |
dc.subject.other | TCE Co-metabolism | en_US |
dc.subject.other | Toluene Oxidizing Bacteria | en_US |
dc.title | Effect of carbon starvation on toluene degradation activity by toluene monooxygenase-expressing bacteria | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Civil and Environmental Engineering, University of Michigan, Ann Arbor, MI 48109-2125, USA, | en_US |
dc.contributor.affiliationum | Department of Civil and Environmental Engineering, University of Michigan, Ann Arbor, MI 48109-2125, USA, ; Department of Civil and Environmental Engineering, Tufts University, Medford, MA 02155, USA, | en_US |
dc.contributor.affiliationother | School of Civil and Environmental Engineering, Yonsei University, Shinchon-dong 134, Seodaemoon-gu, 120-749, Seoul, Republic of Korea, | en_US |
dc.contributor.affiliationother | Biotechnology Center for Agriculture and the Environment, Rutgers University, New Brunswick, NJ 08901-8520, USA, ; Department of Environmental Sciences, Cooks College, Rutgers University, New Brunswick, NJ 08901-8520, USA, | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 11194968 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/45353/1/10532_2005_Article_9014.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/s10532-005-9014-x | en_US |
dc.identifier.source | Biodegradation | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.