Quantitative in vivo receptor binding IV: Detection of muscarinic receptor down-regulation by equilibrium and by tracer kinetic methods
dc.contributor.author | Frey, Kirk A. | en_US |
dc.contributor.author | Ciliax, Brian J. | en_US |
dc.contributor.author | Agranoff, Bernard W. | en_US |
dc.date.accessioned | 2006-09-11T16:01:18Z | |
dc.date.available | 2006-09-11T16:01:18Z | |
dc.date.issued | 1991-09 | en_US |
dc.identifier.citation | Frey, K. A.; Ciliax, B.; Agranoff, B. W.; (1991). "Quantitative in vivo receptor binding IV: Detection of muscarinic receptor down-regulation by equilibrium and by tracer kinetic methods." Neurochemical Research 16(9): 1017-1023. <http://hdl.handle.net/2027.42/45405> | en_US |
dc.identifier.issn | 0364-3190 | en_US |
dc.identifier.issn | 1573-6903 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/45405 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=1784328&dopt=citation | en_US |
dc.description.abstract | Newly-developed methods for estimation of in vivo binding to neurotransmitter receptors should enable the detection and quantification of physiologic or pathologic changes in receptor numbers. In the present study, both equilibrium and kinetic experimental strategies for in vivo muscarinic receptor determination were applied to the detection of receptor changes induced by chronic inhibition of acetylcholinesterase in the rat. Following one week of treatment, in vitro receptor autoradiography utilizing [ 3 H]scopolamine revealed significant losses of muscarinic binding in the cerebral cortex, hippocampus, striatum and in cranial nerve motor nuclei. The in vivo distribution of [ 3 H]scopolamine, following infusion to approach equilibrium binding in the brain, revealed reductions in binding which paralleled the pattern and magnitude of changes detected in vitro. A simplified tracer kinetic estimation following bolus injection of the ligand also detected substantial reductions in forebrain muscarinic receptor binding. These results indicate the feasibility of detecting receptor changes underlying neuropathologic conditions in vivo, and suggest that either equilibrium or kinetic experimental approaches may be extended to clinical research applications with the use of positron or single-photon emission tomography. | en_US |
dc.format.extent | 730721 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Kluwer Academic Publishers-Plenum Publishers; Plenum Publishing Corporation ; Springer Science+Business Media | en_US |
dc.subject.other | Biomedicine | en_US |
dc.subject.other | Neurosciences | en_US |
dc.subject.other | Biochemistry, General | en_US |
dc.subject.other | In Vivo Binding, Muscarinic Receptor, PET (Positron Emission Tomography) | en_US |
dc.subject.other | Neurology | en_US |
dc.subject.other | Scopolamine | en_US |
dc.subject.other | Autoradiography | en_US |
dc.title | Quantitative in vivo receptor binding IV: Detection of muscarinic receptor down-regulation by equilibrium and by tracer kinetic methods | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Psychology | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Internal Medicine and Specialties | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Social Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Internal Medicine (Division of Nuclear Medicine), The University of Michigan, 48109, Ann Arbor, MI; Department of Neurology, The University of Michigan, 48109, Ann Arbor, MI; The Mental Health Research Institute, The University of Michigan, 48109, Ann Arbor, MI; 1500 E. Medical Center Dr., BIG 421/0028 University Hospital, 48109-0028, Ann Arbor, MI | en_US |
dc.contributor.affiliationum | Department of Neurology, The University of Michigan, 48109, Ann Arbor, MI | en_US |
dc.contributor.affiliationum | The Mental Health Research Institute, The University of Michigan, 48109, Ann Arbor, MI | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 1784328 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/45405/1/11064_2004_Article_BF00965845.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/BF00965845 | en_US |
dc.identifier.source | Neurochemical Research | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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