A rapid attenuation of muscarinic agonist stimulated phosphoinositide hydrolysis precedes receptor sequestration in human SH-SY-5Y neuroblastoma cells
dc.contributor.author | McEwen, Edward L. | en_US |
dc.contributor.author | Slowiejko, Diana M. | en_US |
dc.contributor.author | Fisher, Stephen K. | en_US |
dc.date.accessioned | 2006-09-11T16:01:31Z | |
dc.date.available | 2006-09-11T16:01:31Z | |
dc.date.issued | 1994-05 | en_US |
dc.identifier.citation | Fisher, Stephen K.; Slowiejko, Diana M.; McEwen, Edward L.; (1994). "A rapid attenuation of muscarinic agonist stimulated phosphoinositide hydrolysis precedes receptor sequestration in human SH-SY-5Y neuroblastoma cells." Neurochemical Research 19(5): 549-554. <http://hdl.handle.net/2027.42/45408> | en_US |
dc.identifier.issn | 1573-6903 | en_US |
dc.identifier.issn | 0364-3190 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/45408 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=8065510&dopt=citation | en_US |
dc.description.abstract | Agonist occupancy of muscarinic cholinergic receptors in human SH-SY-5Y neuroblastoma cells elicited two kinetically distinct phases of phosphoinositide hydrolysis when monitored by either an increased mass of inositol 1,4,5-trisphosphate, or the accumulation of a total inositol phosphate fraction. Within 5s of the addition of the muscarinic agonist, oxotremorine-M, the phosphoinositide pool was hydrolyzed at a maximal rate of 9.5%/min. This initial phase of phosphoinositide hydrolysis was short-lived (t 1/2 =14s) and after 60s of agonist exposure, the rate of inositol lipid breakdown had declined to a steady state level of 3.4%/min which was then maintained for at least 5–10 min. This rapid, but partial, attenuation of muscarinic receptor stimulated phosphoinositide hydrolysis occurred prior to the agonist-induced internalization of muscarinic receptors. | en_US |
dc.format.extent | 543827 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Kluwer Academic Publishers-Plenum Publishers; Plenum Publishing Corporation ; Springer Science+Business Media | en_US |
dc.subject.other | Desensitization | en_US |
dc.subject.other | Inositol 1,4,5-trisphosphate | en_US |
dc.subject.other | Neurosciences | en_US |
dc.subject.other | Neurology | en_US |
dc.subject.other | Biochemistry, General | en_US |
dc.subject.other | Phosphoinositide Hydrolysis | en_US |
dc.subject.other | SH-SY-5Y Neuroblastoma | en_US |
dc.subject.other | Muscarinic Cholinergic Receptor | en_US |
dc.subject.other | Biomedicine | en_US |
dc.title | A rapid attenuation of muscarinic agonist stimulated phosphoinositide hydrolysis precedes receptor sequestration in human SH-SY-5Y neuroblastoma cells | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Psychology | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Internal Medicine and Specialties | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Social Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Neuroscience Laboratory, University of Michigan, 1103 E. Huron St., 48104-1687, Ann Arbor, MI; Department of Pharmacology, University of Michigan, 48104-1687, Ann Arbor, MI | en_US |
dc.contributor.affiliationum | Neuroscience Laboratory, University of Michigan, 1103 E. Huron St., 48104-1687, Ann Arbor, MI; Department of Pharmacology, University of Michigan, 48104-1687, Ann Arbor, MI | en_US |
dc.contributor.affiliationum | Neuroscience Laboratory, University of Michigan, 1103 E. Huron St., 48104-1687, Ann Arbor, MI | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 8065510 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/45408/1/11064_2004_Article_BF00971329.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/BF00971329 | en_US |
dc.identifier.source | Neurochemical Research | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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