Protein Kinase Inhibitors Block Neurite Outgrowth from Explants of Goldfish Retina
dc.contributor.author | Agranoff, Bernard W. | en_US |
dc.contributor.author | Heacock, Anne M. | en_US |
dc.date.accessioned | 2006-09-11T16:01:49Z | |
dc.date.available | 2006-09-11T16:01:49Z | |
dc.date.issued | 1997-10 | en_US |
dc.identifier.citation | Heacock, Anne M.; Agranoff, Bernard W.; (1997). "Protein Kinase Inhibitors Block Neurite Outgrowth from Explants of Goldfish Retina." Neurochemical Research 22(10): 1179-1185. <http://hdl.handle.net/2027.42/45412> | en_US |
dc.identifier.issn | 0364-3190 | en_US |
dc.identifier.issn | 1573-6903 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/45412 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=9342720&dopt=citation | en_US |
dc.description.abstract | A role for protein phosphorylation in the process of neurite outgrowth has been inferred from many studies of the effects of protein kinase inhibitors and activators on cultured neurotumor cells and primary neuronal cells from developing brain or ganglia. Here we re-examine this issue, using a culture system derived from a fully differentiated neuronal system undergoing axonal regeneration—the explanted goldfish retina following optic nerve crush. Of the relatively non-selective protein kinase inhibitors employed, H7, staurosporine and K 252a were found to block neurite outgrowth, whereas HA1004 had no effect, a result which appears to rule out a critical role for protein kinase A. The more selective protein kinase C inhibitors, sphingosine, calphostin C and Ro-31-8220 were all inhibitory, as was prolonged treatment with phorbol ester and the protein phosphatase inhibitor okadaic acid. These results are in support of a role for protein kinase C in axonal regrowth. | en_US |
dc.format.extent | 1486596 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Kluwer Academic Publishers-Plenum Publishers; Plenum Publishing Corporation ; Springer Science+Business Media | en_US |
dc.subject.other | Goldfish Retina | en_US |
dc.subject.other | Neurology | en_US |
dc.subject.other | Protein Kinase | en_US |
dc.subject.other | Biomedicine | en_US |
dc.subject.other | Neurosciences | en_US |
dc.subject.other | Biochemistry, General | en_US |
dc.subject.other | Neurite Outgrowth | en_US |
dc.subject.other | Regeneration | en_US |
dc.title | Protein Kinase Inhibitors Block Neurite Outgrowth from Explants of Goldfish Retina | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Psychology | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Internal Medicine and Specialties | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Social Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Neuroscience Laboratory, University of Michigan, Ann Arbor, MI, 48104-1687 | en_US |
dc.contributor.affiliationum | Neuroscience Laboratory, University of Michigan, Ann Arbor, MI, 48104-1687 | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 9342720 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/45412/1/11064_2004_Article_413024.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1023/A:1021916509858 | en_US |
dc.identifier.source | Neurochemical Research | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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