Regulation of Myo-Inositol Homeostasis in Differentiated Human NT2-N Neurons
dc.contributor.author | Novak, James E. | en_US |
dc.contributor.author | Agranoff, Bernard W. | en_US |
dc.contributor.author | Fisher, Stephen K. | en_US |
dc.date.accessioned | 2006-09-11T16:02:19Z | |
dc.date.available | 2006-09-11T16:02:19Z | |
dc.date.issued | 2000-05 | en_US |
dc.identifier.citation | Novak, James E.; Agranoff, Bernard W.; Fisher, Stephen K.; (2000). "Regulation of Myo-Inositol Homeostasis in Differentiated Human NT2-N Neurons." Neurochemical Research 25(5): 561-566. <http://hdl.handle.net/2027.42/45419> | en_US |
dc.identifier.issn | 1573-6903 | en_US |
dc.identifier.issn | 0364-3190 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/45419 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=10905616&dopt=citation | en_US |
dc.description.abstract | We have investigated the possible role of second messengers on inositol homeostasis in NT2-N cells, human central nervous system neurons obtained by terminal differentiation of teratocarcinoma precursors. Uptake of inositol into NT2-N neurons was inhibited ∼10% by protein kinase C (PKC) activation but was unaffected by either the presence of cyclic nucleotide analogs or changes in the intracellular concentration of Ca 2+ . Efflux of inositol from NT2-N neurons was enhanced in hypotonic buffer but virtually eliminated by inclusion of the Cl − channel blocker 4,4′-diisothiocyanatostilbene-2,2′-disulfonic acid, a result which indicates the involvement of a volume-sensitive organic osmolyte-anion channel. Volume-sensitive inositol efflux was stimulated ∼30% following activation of PKC or elevation of the cytosolic Ca 2+ concentration but was unaffected by protein kinase A activation. These results suggest that whereas inositol uptake into NT2-N neurons is relatively refractory to regulation, volume-sensitive inositol efflux may be significantly affected by intracellular signaling events. | en_US |
dc.format.extent | 56645 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Kluwer Academic Publishers-Plenum Publishers; Plenum Publishing Corporation ; Springer Science+Business Media | en_US |
dc.subject.other | Second Messenger | en_US |
dc.subject.other | Calcium | en_US |
dc.subject.other | Neurosciences | en_US |
dc.subject.other | Biochemistry, General | en_US |
dc.subject.other | Biomedicine | en_US |
dc.subject.other | Neurology | en_US |
dc.subject.other | Inositol Uptake | en_US |
dc.subject.other | Inositol Efflux | en_US |
dc.subject.other | Neuron | en_US |
dc.subject.other | Protein Kinase C | en_US |
dc.title | Regulation of Myo-Inositol Homeostasis in Differentiated Human NT2-N Neurons | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Psychology | en_US |
dc.subject.hlbsecondlevel | Neurosciences | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Internal Medicine and Specialties | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Social Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Neuroscience Laboratory and Mental Health Research Institute, University of Michigan, Ann Arbor, MI; Department of Pharmacology, University of Michigan, Ann Arbor, MI | en_US |
dc.contributor.affiliationum | Neuroscience Laboratory and Mental Health Research Institute, University of Michigan, Ann Arbor, MI; University of Michigan, Ann Arbor, MI | en_US |
dc.contributor.affiliationum | Neuroscience Laboratory and Mental Health Research Institute, University of Michigan, Ann Arbor, MI; University of Michigan, Ann Arbor, MI | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 10905616 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/45419/1/11064_2004_Article_225540.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1023/A:1007538431486 | en_US |
dc.identifier.source | Neurochemical Research | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.