Herpes simplex virus thymidine kinase gene is stably maintained and expressed in cells transformed by protoplast fusion
dc.contributor.author | Levine, Myron | en_US |
dc.contributor.author | Sandri-Goldin, Rozanne M. | en_US |
dc.contributor.author | Goldin, Alan L. | en_US |
dc.contributor.author | Glorioso, Joseph C. | en_US |
dc.date.accessioned | 2006-09-11T16:10:14Z | |
dc.date.available | 2006-09-11T16:10:14Z | |
dc.date.issued | 1984-03 | en_US |
dc.identifier.citation | Sandri-Goldin, Rozanne M.; Goldin, Alan L.; Glorioso, Joseph; Levine, Myron; (1984). "Herpes simplex virus thymidine kinase gene is stably maintained and expressed in cells transformed by protoplast fusion." Somatic Cell and Molecular Genetics 10(2): 129-138. <http://hdl.handle.net/2027.42/45531> | en_US |
dc.identifier.issn | 0740-7750 | en_US |
dc.identifier.issn | 1572-9931 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/45531 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=6324392&dopt=citation | en_US |
dc.description.abstract | We examined a series of transformed cell lines resulting from transfer of the herpes simplex virus type 1 thymidine kinase gene to Ltk − cells by protoplast fusion gene transfer. We show that multiple copies of the transforming plasmid DNA, ranging from a minimum of two to greater than 20, were present in one or at most a few integration sites in each cell line. The TK + phenotype was stable in five independent transformed cell lines after growth in nonselective medium for over a year. Transforming plasmid DNA was stable in one cell line containing from two to five copies after a year of growth in nonselective medium. In another cell line initially containing about 20 copies, the transforming DNA became rearranged soon after growth to mass culture, resulting in a decrease to two to five copies which then remained stably maintained. This suggests that TK + transformants resulting from protoplast fusion are stable when the input DNA has integrated in a relatively low copy number. | en_US |
dc.format.extent | 1250595 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Kluwer Academic Publishers-Plenum Publishers; Plenum Publishing Corporation ; Springer Science+Business Media | en_US |
dc.subject.other | Animal Anatomy / Morphology / Histology | en_US |
dc.subject.other | Biomedicine | en_US |
dc.subject.other | Biochemistry, General | en_US |
dc.subject.other | Human Genetics | en_US |
dc.subject.other | Plant Sciences | en_US |
dc.title | Herpes simplex virus thymidine kinase gene is stably maintained and expressed in cells transformed by protoplast fusion | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Unit for Laboratory Animal Medicine, University of Michigan, 48109, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Department of Human Genetics, University of Michigan, 48109, Ann Arbor, Michigan; Department of Microbiology, University of California, 92717, Irvine, California | en_US |
dc.contributor.affiliationum | Department of Human Genetics, University of Michigan, 48109, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Department of Human Genetics, University of Michigan, 48109, Ann Arbor, Michigan; Department of Chemistry, California Institute of Technology, 91125, Pasadena, California | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 6324392 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/45531/1/11188_2005_Article_BF01534902.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/BF01534902 | en_US |
dc.identifier.source | Somatic Cell and Molecular Genetics | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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