Paradoxical regulation of human argininosuccinate synthetase cDNA minigene in opposition to endogenous gene: Evidence for intragenic control sequences
dc.contributor.author | Pogulis, Robert J. | en_US |
dc.contributor.author | Freytag, Svend O. | en_US |
dc.contributor.author | Boyce, Frederick M. | en_US |
dc.date.accessioned | 2006-09-11T16:10:44Z | |
dc.date.available | 2006-09-11T16:10:44Z | |
dc.date.issued | 1989-03 | en_US |
dc.identifier.citation | Boyce, Frederick M.; Pogulis, Robert J.; Freytag, Svend O.; (1989). "Paradoxical regulation of human argininosuccinate synthetase cDNA minigene in opposition to endogenous gene: Evidence for intragenic control sequences." Somatic Cell and Molecular Genetics 15(2): 123-129. <http://hdl.handle.net/2027.42/45538> | en_US |
dc.identifier.issn | 1572-9931 | en_US |
dc.identifier.issn | 0740-7750 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/45538 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=2538928&dopt=citation | en_US |
dc.description.abstract | Human somatic cell variants resistant to the arginine analog, canavanine, express 200-fold increased levels of argininosuccinate synthetase (AS) mRNA as compared to parental cells. In this study we examined whether AS cDNA sequences contain cis- acting regulatory elements that are involved in the induction of AS mRNA in canavanine-resistant cells. Minigene constructs containing AS cDNA sequences under the transcriptional control of a viral promoter were stably transfected into the human squamous cell carcinoma line, RPMI 2650. Upon conversion of cells to canavanine-resistance, expression of the endogenous AS gene increased by two orders of magnitude as expected. Surprisingly, however, expression of AS cDNA minigenes decreased 10- to 15- fold in canavanine-resistant cell variants. The observed down-modulation of AS cDNA minigene expression was dependent upon a concomitant induction of the endogenous AS gene and not simply expression of the canavanine-resistant phenotype. This paradoxical regulation was specific for AS gene sequences since a minigene containing the neomycin-resistance gene in place of AS cDNA sequences failed to regulate. Furthermore, minigenes lacking a substantial portion of the AS cDNA also failed to exhibit the down-modulation. These findings suggest that expression of the human AS gene is regulated by a specific and limiting, positively-acting , trans- acting mechanism in canavanine-resistant cells and that exogenous AS cDNA (mRNA) sequences can compete for this mechanism . | en_US |
dc.format.extent | 895770 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Kluwer Academic Publishers-Plenum Publishers; Plenum Publishing Corporation ; Springer Science+Business Media | en_US |
dc.subject.other | Plant Sciences | en_US |
dc.subject.other | Biomedicine | en_US |
dc.subject.other | Human Genetics | en_US |
dc.subject.other | Biochemistry, General | en_US |
dc.subject.other | Animal Anatomy / Morphology / Histology | en_US |
dc.title | Paradoxical regulation of human argininosuccinate synthetase cDNA minigene in opposition to endogenous gene: Evidence for intragenic control sequences | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry and Program in Cellular and Molecular Biology, University of Michigan Medical School, 48109-0606, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry and Program in Cellular and Molecular Biology, University of Michigan Medical School, 48109-0606, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | Department of Biological Chemistry and Program in Cellular and Molecular Biology, University of Michigan Medical School, 48109-0606, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 2538928 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/45538/1/11188_2005_Article_BF01535072.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/BF01535072 | en_US |
dc.identifier.source | Somatic Cell and Molecular Genetics | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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