Neurofibromatosis type 1 gene product (neurofibromin) associates with microtubules
dc.contributor.author | Gregory, Paula E. | en_US |
dc.contributor.author | Gutmann, David H. | en_US |
dc.contributor.author | Mitchell, Anna L. | en_US |
dc.contributor.author | Park, Soochul | en_US |
dc.contributor.author | Boguski, Mark | en_US |
dc.contributor.author | Jacks, Tyler | en_US |
dc.contributor.author | Wood, Deborah L. | en_US |
dc.contributor.author | Jove, Richard | en_US |
dc.contributor.author | Collins, Francis S. | en_US |
dc.date.accessioned | 2006-09-11T16:11:09Z | |
dc.date.available | 2006-09-11T16:11:09Z | |
dc.date.issued | 1993-05 | en_US |
dc.identifier.citation | Gregory, Paula E.; Gutmann, David H.; Mitchell, Anna; Park, Soochul; Boguski, Mark; Jacks, Tyler; Wood, Deborah L.; Jove, Richard; Collins, Francis S.; (1993). "Neurofibromatosis type 1 gene product (neurofibromin) associates with microtubules." Somatic Cell and Molecular Genetics 19(3): 265-274. <http://hdl.handle.net/2027.42/45544> | en_US |
dc.identifier.issn | 0740-7750 | en_US |
dc.identifier.issn | 1572-9931 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/45544 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=8332934&dopt=citation | en_US |
dc.description.abstract | The neurofibromatosis type 1 (NF1) gene was recently identified by positional cloning and found to encode a protein with structural and functional homology to mammalian and yeast GTPase-activating proteins (GAPs). Using antibodies directed against the NF1 gene product, a protein of ∼250kDa was identified and termed neurofibromin. Double-indirect immunofluorescent labeling with anti-neurofibromin and anti-tubulin antibodies demonstrates that neurofibromin associates with cytoplasmic microtubules. Immunoblotting of microtubule-enriched cytoplasmic fractions, using antibodies generated against neurofibromin, shows that neurofibromin copurifies with microtubules. When portions of neurofibromin are expressed in Sf9 insect cells they associate with polymerized microtubules; furthermore, the critical residues for this interaction reside within the GAP-related domain of neurofibromin. The unexpected association of neurofibromin with microtubules suggests that neurofibromin is involved in microtubule-mediated intracellullar signal transduction pathways. | en_US |
dc.format.extent | 1053449 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Kluwer Academic Publishers-Plenum Publishers; Plenum Publishing Corporation ; Springer Science+Business Media | en_US |
dc.subject.other | Biochemistry, General | en_US |
dc.subject.other | Biomedicine | en_US |
dc.subject.other | Animal Anatomy / Morphology / Histology | en_US |
dc.subject.other | Human Genetics | en_US |
dc.subject.other | Plant Sciences | en_US |
dc.title | Neurofibromatosis type 1 gene product (neurofibromin) associates with microtubules | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | The Departments of Internal Medicine, Human Genetics, Neurology and Microbiology and Immunology, The University of Michigan Medical Center and The Howard Hughes Medical Institute, The University of Michigan, 48109, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | The Departments of Internal Medicine, Human Genetics, Neurology and Microbiology and Immunology, The University of Michigan Medical Center and The Howard Hughes Medical Institute, The University of Michigan, 48109, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | The Departments of Internal Medicine, Human Genetics, Neurology and Microbiology and Immunology, The University of Michigan Medical Center and The Howard Hughes Medical Institute, The University of Michigan, 48109, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | The Departments of Internal Medicine, Human Genetics, Neurology and Microbiology and Immunology, The University of Michigan Medical Center and The Howard Hughes Medical Institute, The University of Michigan, 48109, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | The Departments of Internal Medicine, Human Genetics, Neurology and Microbiology and Immunology, The University of Michigan Medical Center and The Howard Hughes Medical Institute, The University of Michigan, 48109, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | The Departments of Internal Medicine, Human Genetics, Neurology and Microbiology and Immunology, The University of Michigan Medical Center and The Howard Hughes Medical Institute, The University of Michigan, 48109, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationum | The Departments of Internal Medicine, Human Genetics, Neurology and Microbiology and Immunology, The University of Michigan Medical Center and The Howard Hughes Medical Institute, The University of Michigan, 48109, Ann Arbor, Michigan | en_US |
dc.contributor.affiliationother | Center for Cancer Research, Massachusetts Institute of Technology, 40 Ames Street, 02139, Cambridge, Massachusetts | en_US |
dc.contributor.affiliationother | National Center for Biotechnology Information, NIH, 20894, Bethesda, Maryland | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 8332934 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/45544/1/11188_2005_Article_BF01233074.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/BF01233074 | en_US |
dc.identifier.source | Somatic Cell and Molecular Genetics | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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