Targeted retroviral gene transfer into the rat biliary tract
dc.contributor.author | Raper, Steven E. | en_US |
dc.contributor.author | Wilson, James M. | en_US |
dc.contributor.author | Cabrera, Jesus A. | en_US |
dc.date.accessioned | 2006-09-11T16:11:22Z | |
dc.date.available | 2006-09-11T16:11:22Z | |
dc.date.issued | 1996-01 | en_US |
dc.identifier.citation | Cabrera, Jesus A.; Wilson, James M.; Raper, Steven E.; (1996). "Targeted retroviral gene transfer into the rat biliary tract." Somatic Cell and Molecular Genetics 22(1): 21-29. <http://hdl.handle.net/2027.42/45547> | en_US |
dc.identifier.issn | 1572-9931 | en_US |
dc.identifier.issn | 0740-7750 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/45547 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=8643991&dopt=citation | en_US |
dc.description.abstract | The ability to induce proliferation by temporary duct ligation suggested an hypothesis that retrovirus-mediated gene transfer into cells of the biliary tract could be accomplished. The time course of histologic changes, incorporation of 3 H-thymidine and immunofluorescent staining with a monoclonal antibody to cytokeratin-19 (a marker for differentiated bile ducts) was studied in male Fischer F344 rats. A recombinant Gibbon ape leukemia virus (GALV), containing a gene encoding Escherichia coli β-galactosidase was next introduced into 24 hr obstructed bile ducts. Gene transfer was maximal when virus was exposed to the obstructed duct for 12 hr (∼0.1%). The majority of X-gal positive cells were in cytokeratin-19 negative peribiliary tissues, which had the appearance of newly forming bile ducts. The data suggest that cells targeted by retroviral infection of the obstructed rat bile duct may be a precursor of mature, fully differentiated biliary epithelium. | en_US |
dc.format.extent | 4097680 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Kluwer Academic Publishers-Plenum Publishers; Plenum Publishing Corporation ; Springer Science+Business Media | en_US |
dc.subject.other | Plant Sciences | en_US |
dc.subject.other | Human Genetics | en_US |
dc.subject.other | Biomedicine | en_US |
dc.subject.other | Biochemistry, General | en_US |
dc.subject.other | Animal Anatomy / Morphology / Histology | en_US |
dc.title | Targeted retroviral gene transfer into the rat biliary tract | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | University of Michigan Medical School, Room 206, Wistar Institute, 36th and Spruce Streets, 19104-4268, Philadelphia, Pennsylvania | en_US |
dc.contributor.affiliationother | Department of Molecular and Cellular Engineering, Department of Surgery, University of Pennsylvania School of Medicine, Pennsylvania, USA | en_US |
dc.contributor.affiliationother | Institute for Human Gene Therapy, Room 206, Wistar Institute, 36th and Spruce Streets, 19104-4268, Philadelphia, Pennsylvania | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 8643991 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/45547/1/11188_2006_Article_BF02374373.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/BF02374373 | en_US |
dc.identifier.source | Somatic Cell and Molecular Genetics | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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