Mutagen-induced diploid human lymphoblast variants containing altered hypoxanthine guanine phosphoribosyl transferase

Show simple item record

dc.contributor.author Littlefield, J. W. en_US
dc.contributor.author Kelley, William N. en_US
dc.contributor.author Epstein, J. en_US
dc.contributor.author Leyva, A. en_US
dc.date.accessioned 2006-09-11T16:11:39Z
dc.date.available 2006-09-11T16:11:39Z
dc.date.issued 1977-03 en_US
dc.identifier.citation Epstein, J.; Leyva, A.; Kelley, W. N.; Littlefield, J. W.; (1977). "Mutagen-induced diploid human lymphoblast variants containing altered hypoxanthine guanine phosphoribosyl transferase." Somatic Cell Genetics 3(2): 135-148. <http://hdl.handle.net/2027.42/45551> en_US
dc.identifier.issn 1572-9931 en_US
dc.identifier.issn 0098-0366 en_US
dc.identifier.uri http://hdl.handle.net/2027.42/45551
dc.identifier.uri http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=605378&dopt=citation en_US
dc.description.abstract The human lymphoblast line MGL8 was treated with HAT and subsequently “mutagenized” with EMS (200 μg/ml) to give 15% survival, and 6-thioguanine-resistant cells were selected by cloning in soft agarose containing the drug (1 μg/ml). Eighteen sublines of independently derived resistant clones were isolated and studied in detail. One subline had a low residual HGPRT activity of about 1% of the parental cells. The HGPRT of this subline had a higher K m for PRPP, was more sensitive to heat, and was degraded faster by trypsin than the enzyme in extracts of MGL8 cells. This resistant subline and three others contained CRM levels of 1-38%, compared to the wild-type, so they probably represent true structural mutants of the HGPRT gene. All the variants maintained the karyotype of the parental line (46, XY, 6p − ). en_US
dc.format.extent 705757 bytes
dc.format.extent 3115 bytes
dc.format.mimetype application/pdf
dc.format.mimetype text/plain
dc.language.iso en_US
dc.publisher Kluwer Academic Publishers-Plenum Publishers; Plenum Publishing Corporation ; Springer Science+Business Media en_US
dc.subject.other Plant Sciences en_US
dc.subject.other Human Genetics en_US
dc.subject.other Biomedicine en_US
dc.subject.other Biochemistry, General en_US
dc.subject.other Animal Anatomy / Morphology / Histology en_US
dc.title Mutagen-induced diploid human lymphoblast variants containing altered hypoxanthine guanine phosphoribosyl transferase en_US
dc.subject.hlbsecondlevel Natural Resources and Environment en_US
dc.subject.hlbsecondlevel Molecular, Cellular and Developmental Biology en_US
dc.subject.hlbsecondlevel Ecology and Evolutionary Biology en_US
dc.subject.hlbtoplevel Health Sciences en_US
dc.subject.hlbtoplevel Science en_US
dc.description.peerreviewed Peer Reviewed en_US
dc.contributor.affiliationum Department of Internal Medicine, University of Michigan Medical Center, 48104, Ann Arbor, Michigan en_US
dc.contributor.affiliationum Department of Internal Medicine, University of Michigan Medical Center, 48104, Ann Arbor, Michigan en_US
dc.contributor.affiliationother Department of Pediatrics, Johns Hopkins University School of Medicine, 21205, Baltimore, Maryland; Laboratory of Experimental Oncology, Indiana University Medical Centre, 46202, Indianapolis, Indina en_US
dc.contributor.affiliationother Department of Pediatrics, Johns Hopkins University School of Medicine, 21205, Baltimore, Maryland en_US
dc.contributor.affiliationumcampus Ann Arbor en_US
dc.identifier.pmid 605378 en_US
dc.description.bitstreamurl http://deepblue.lib.umich.edu/bitstream/2027.42/45551/1/11188_2005_Article_BF01551810.pdf en_US
dc.identifier.doi http://dx.doi.org/10.1007/BF01551810 en_US
dc.identifier.source Somatic Cell Genetics en_US
dc.owningcollname Interdisciplinary and Peer-Reviewed
 Show simple item record

This item appears in the following Collection(s)


Search Deep Blue

Advanced Search

Browse by

My Account

Information

Coming Soon


MLibrary logo