View Item 

Show simple item record

Isolation and characterization of a Forssman antigen-binding lectin from velvet bean ( Mucuna derringiana ) seeds
dc.contributor.authorMo, Hanqingen_US
dc.contributor.authorGoldstein, Irwin J.en_US
dc.date.accessioned2006-09-11T16:19:51Z
dc.date.available2006-09-11T16:19:51Z
dc.date.issued1994-10en_US
dc.identifier.citationMo, Hanqing; Goldstein, Irwin J.; (1994). "Isolation and characterization of a Forssman antigen-binding lectin from velvet bean ( Mucuna derringiana ) seeds." Glycoconjugate Journal 11(5): 424-431. <http://hdl.handle.net/2027.42/45669>en_US
dc.identifier.issn0282-0080en_US
dc.identifier.issn1573-4986en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/45669
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=7696847&dopt=citationen_US
dc.description.abstractA Forssman antigen (GalNAcα1-3GalNAcβ1-3Galα1-4Galβ1-4Glcβ1-1Cer)-binding lectin has been purified from velvet bean ( Mucuna derringiana ) seeds by a combination of affinity chromatography and reversed phase HPLC. This lectin agglutinates both native and trypsin-treated sheep erythrocytes as well as trypsinized rabbit erythrocytes, but neither native rabbit nor human erythrocytes, irrespective of blood group type. SDS-PAGE and gel filtration chromatography reveal the lectin to be a homodimer consisting of two 54 kDa subunits linked by non-covalent bonds. The results obtained by quantitative precipitation, haemagglutination inhibition and TLC overlay assays indicate that the Mucuna lectin specifically recognizes Forssman antigen and Forssman disaccharide (GalNAcα1-3GalNAc)-related structures.en_US
dc.format.extent1020501 bytes
dc.format.extent3115 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherKluwer Academic Publishers; Chapman & Hall ; Springer Science+Business Mediaen_US
dc.subject.otherLife Sciencesen_US
dc.subject.otherPathologyen_US
dc.subject.otherBiochemistry, Generalen_US
dc.subject.otherMucuna ( Mucuna Derringiana ) Lectinen_US
dc.subject.otherVelvet Bean Lectinen_US
dc.subject.otherHaemagglutininen_US
dc.subject.otherForssman Antigen Binding Lectinen_US
dc.titleIsolation and characterization of a Forssman antigen-binding lectin from velvet bean ( Mucuna derringiana ) seedsen_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelChemistryen_US
dc.subject.hlbsecondlevelDentistryen_US
dc.subject.hlbsecondlevelPublic Healthen_US
dc.subject.hlbsecondlevelChemical Engineeringen_US
dc.subject.hlbsecondlevelBiological Chemistryen_US
dc.subject.hlbtoplevelScienceen_US
dc.subject.hlbtoplevelEngineeringen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDepartment of Biological Chemistry, University of Michigan, 48109, Ann Arbor, Michigan, USAen_US
dc.contributor.affiliationumDepartment of Biological Chemistry, University of Michigan, 48109, Ann Arbor, Michigan, USAen_US
dc.contributor.affiliationumcampusAnn Arboren_US
dc.identifier.pmid7696847en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/45669/1/10719_2004_Article_BF00731278.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1007/BF00731278en_US
dc.identifier.sourceGlycoconjugate Journalen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


Files in this item

Name:
10719_2004_Article_BF00731278.pdf
Size:
996.5KB
Format:
PDF
View/Open

Show simple item record

Remediation of Harmful Language

The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.

Accessibility

If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.