Estimates of free and bound indole-3-acetic acid and zeatin levels in relation to regulation of apical dominance and tiller release in oat shoots
dc.contributor.author | Harrison, Marcia A. | en_US |
dc.contributor.author | Kaufman, Peter B. | en_US |
dc.date.accessioned | 2006-09-11T17:09:49Z | |
dc.date.available | 2006-09-11T17:09:49Z | |
dc.date.issued | 1983-08 | en_US |
dc.identifier.citation | Harrison, Marcia A.; Kaufman, Peter B.; (1983). "Estimates of free and bound indole-3-acetic acid and zeatin levels in relation to regulation of apical dominance and tiller release in oat shoots." Journal of Plant Growth Regulation 2 (1-3): 215-223. <http://hdl.handle.net/2027.42/45928> | en_US |
dc.identifier.issn | 0721-7595 | en_US |
dc.identifier.issn | 1435-8107 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/45928 | |
dc.description.abstract | Oat stem segments containing quiescent lateral (tiller) buds during times of strong apical dominance, and growing buds released from this inhibition, were collected for analysis of native auxin and cytokinins. Free IAA and IAA conjugates were determined by a 14 C-IAA and 14 C-IBA double isotope dilution assay. Free zeatin (Z), zeatin riboside (Z-r), and their glucoside conjugates were purified from butanol-soluble fractions by means of a cellulose phosphate exchanger and thin-layer chromatography. Hormones were analyzed by gas chromatography and mass spectrometry (GC-MS). Results of these analyses indicate that changes in free and bound IAA within the stem do not correlate well with the release of tiller buds (as brought about by decapitation, gravistimulation, or the emergence of the inflorescence). However, increases in Z-r levels are well correlated with tiller release. The glucoside conjugate of Z-r may act as a storage form of cytokinin in quiescent tiller buds. In light of these results, we find that the auxin-cytokinin ratio in oat stem segments is shifted during tiller | en_US |
dc.format.extent | 416319 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Springer-Verlag; Springer-Verlag New York Inc. | en_US |
dc.subject.other | Life Sciences | en_US |
dc.subject.other | Plant Sciences | en_US |
dc.subject.other | Cell Biology | en_US |
dc.subject.other | Agriculture | en_US |
dc.subject.other | Forestry | en_US |
dc.title | Estimates of free and bound indole-3-acetic acid and zeatin levels in relation to regulation of apical dominance and tiller release in oat shoots | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Departments of Botany and Cellular and Molecular Biology, University of Michigan, 48109, Ann Arbor, Michigan, USA | en_US |
dc.contributor.affiliationum | Departments of Botany and Cellular and Molecular Biology, University of Michigan, 48109, Ann Arbor, Michigan, USA | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/45928/1/344_2005_Article_BF02042250.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/BF02042250 | en_US |
dc.identifier.source | Journal of Plant Growth Regulation | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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