Determination of ploidy level and nuclear DNA content in blueberry by flow cytometry
dc.contributor.author | Costich, D. E. | en_US |
dc.contributor.author | Ortiz, Rodomiro | en_US |
dc.contributor.author | Meagher, T. R. | en_US |
dc.contributor.author | Bruederle, Leo P. | en_US |
dc.contributor.author | Vorsa, Nicholi | en_US |
dc.date.accessioned | 2006-09-11T17:16:26Z | |
dc.date.available | 2006-09-11T17:16:26Z | |
dc.date.issued | 1993-09 | en_US |
dc.identifier.citation | Costich, D. E.; Ortiz, R.; Meagher, T. R.; Bruederle, L. P.; Vorsa, N.; (1993). "Determination of ploidy level and nuclear DNA content in blueberry by flow cytometry." Theoretical and Applied Genetics 86(8): 1001-1006. <http://hdl.handle.net/2027.42/46013> | en_US |
dc.identifier.issn | 0040-5752 | en_US |
dc.identifier.issn | 1432-2242 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/46013 | |
dc.description.abstract | The technique of DNA flow cytometry was used to study variation in DNA content among different ploidy levels, as well as among diploid species, of Vaccinium section Cyanococcus . In a sample of plants of varying ploidy level, the relative fluorescence intensity (RFI) of nuclei stained with propidium iodide was a function of the number of chromosome sets (x), as represented by the linear equation RFI=3.7x-2.3 (r 2 =95%). The data indicated that DNA flow cytometry could be useful for the determination of ploidy level at the seedling stage in blueberry. They also suggest that “conventional polyploid evolution” has occurred in this section of the genus Vaccinium with an increase in nuclear DNA content concurrent with the increase in chromosome number. The nuclear DNA content of diploid species of Vaccinium section Cyanococcus was estimated from the relationship of the observed RFI to an internal known DNA standard (trout red blood cells). A nested analysis of variance indicated significant variation among species, as well as among populations within species, in nuclear DNA content, although this variation was small compared to the variation among ploidy levels. The variation in nuclear DNA content corresponded to the phylogenetic relationships among species determined from previous studies. | en_US |
dc.format.extent | 630066 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Springer-Verlag | en_US |
dc.subject.other | Plant Genetics & Genomics | en_US |
dc.subject.other | Genome Size | en_US |
dc.subject.other | Plant Biochemistry | en_US |
dc.subject.other | Agriculture | en_US |
dc.subject.other | Life Sciences | en_US |
dc.subject.other | Biotechnology | en_US |
dc.subject.other | Biochemistry, General | en_US |
dc.subject.other | Plant Sciences | en_US |
dc.subject.other | Vaccinium | en_US |
dc.subject.other | Nuclear DNA Content | en_US |
dc.subject.other | Polyploid Evolution | en_US |
dc.title | Determination of ploidy level and nuclear DNA content in blueberry by flow cytometry | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Blueberry and Cranberry Research Center, Cook College, Rutgers University, 08019, Chatsworth, NJ, USA; Biology Department, The University of Michigan-Flint, 48502-2186, MI, USA | en_US |
dc.contributor.affiliationother | Blueberry and Cranberry Research Center, Cook College, Rutgers University, 08019, Chatsworth, NJ, USA | en_US |
dc.contributor.affiliationother | Department of Biological Sciences, Rutgers University, P.O. Box 1059, 08855-1059, Piscataway, NJ, USA | en_US |
dc.contributor.affiliationother | Blueberry and Cranberry Research Center, Cook College, Rutgers University, 08019, Chatsworth, NJ, USA | en_US |
dc.contributor.affiliationother | Department of Biological Sciences, Rutgers University, P.O. Box 1059, 08855-1059, Piscataway, NJ, USA | en_US |
dc.contributor.affiliationumcampus | Flint | en_US |
dc.identifier.pmid | 24194009 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/46013/1/122_2004_Article_BF00211053.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/BF00211053 | en_US |
dc.identifier.source | Theoretical and Applied Genetics | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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