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Aldose reductase in the BB rat: isolation, immunological identification and localization in the retina and peripheral nerve

dc.contributor.authorChakrabarti, Subrataen_US
dc.contributor.authorSima, Anders A. F.en_US
dc.contributor.authorNakajima, T.en_US
dc.contributor.authorYagihashi, S.en_US
dc.contributor.authorGreene, Douglas A.en_US
dc.date.accessioned2006-09-11T17:16:51Z
dc.date.available2006-09-11T17:16:51Z
dc.date.issued1987-04en_US
dc.identifier.citationChakrabarti, S.; Sima, A. A. F.; Nakajima, T.; Yagihashi, S.; Greene, D. A.; (1987). "Aldose reductase in the BB rat: isolation, immunological identification and localization in the retina and peripheral nerve." Diabetologia 30(4): 244-251. <http://hdl.handle.net/2027.42/46019>en_US
dc.identifier.issn0012-186Xen_US
dc.identifier.issn1432-0428en_US
dc.identifier.urihttps://hdl.handle.net/2027.42/46019
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=3109992&dopt=citationen_US
dc.description.abstractAldose reductase was purified from testis of nondiabetic BB rats using DEAE cellulose, hydroxylapatite and sephadex G-100 column chromatography. The molecular weight of the isolated enzyme was found to be 36,500±1000. Antibody against the isolated enzyme was raised in rabbits. It was purified by affinity chromatography, characterised by double immunodiffusion and Western blot analysis and used to localize the enzyme in retina and in peripheral nerve of the BB rat. In the retina, aldose reductase immunoreactivity was seen in the ganglion cells, Müller cell processes, retinal pigment epithelium and in the pericytes and endothelial cells of retinal capillaries. In peripheral nerve, aldose reductase immunoreactivity was found in the paranodal cytoplasm of Schwann cells and in pericytes and endothelial cells of endoneurial capillaries.en_US
dc.format.extent2321351 bytes
dc.format.extent3115 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_US
dc.publisherSpringer-Verlagen_US
dc.subject.otherPeripheral Nerveen_US
dc.subject.otherMedicine & Public Healthen_US
dc.subject.otherInternal Medicineen_US
dc.subject.otherAldose Reductaseen_US
dc.subject.otherHuman Physiologyen_US
dc.subject.otherMetabolic Diseasesen_US
dc.subject.otherBB Raten_US
dc.subject.otherImmunocytochemistryen_US
dc.subject.otherRetinaen_US
dc.titleAldose reductase in the BB rat: isolation, immunological identification and localization in the retina and peripheral nerveen_US
dc.typeArticleen_US
dc.subject.hlbsecondlevelInternal Medicine and Specialtiesen_US
dc.subject.hlbtoplevelHealth Sciencesen_US
dc.description.peerreviewedPeer Revieweden_US
dc.contributor.affiliationumDiabetes Research and Training Center, Department of Medicine, University of Michigan School of Medicine, Ann Arbor, Michigan, USAen_US
dc.contributor.affiliationotherDepartment of Immunology, University of Manitoba, Winnipeg, Manitoba, Canadaen_US
dc.contributor.affiliationotherNeuropathology Research Laboratory, Department of Pathology, University of Manitoba, Winnipeg, Manitoba, Canadaen_US
dc.contributor.affiliationotherNeuropathology Research Laboratory, Department of Pathology, University of Manitoba, Winnipeg, Manitoba, Canadaen_US
dc.contributor.affiliationotherNeuropathology Research Laboratory, Department of Pathology, University of Manitoba, Winnipeg, Manitoba, Canadaen_US
dc.contributor.affiliationumcampusAnn Arboren_US
dc.identifier.pmid3109992en_US
dc.description.bitstreamurlhttp://deepblue.lib.umich.edu/bitstream/2027.42/46019/1/125_2004_Article_BF00270423.pdfen_US
dc.identifier.doihttp://dx.doi.org/10.1007/BF00270423en_US
dc.identifier.sourceDiabetologiaen_US
dc.owningcollnameInterdisciplinary and Peer-Reviewed


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