The effect of feedback regulation and in situ product removal on the conversion of sugar to cycloheximide by Streptomyces griseus
dc.contributor.author | Payne, Gregory F. | en_US |
dc.contributor.author | Wang, Henry Y. | en_US |
dc.date.accessioned | 2006-09-11T17:24:49Z | |
dc.date.available | 2006-09-11T17:24:49Z | |
dc.date.issued | 1989-03 | en_US |
dc.identifier.citation | Payne, Gregory F.; Wang, Henry Y.; (1989). "The effect of feedback regulation and in situ product removal on the conversion of sugar to cycloheximide by Streptomyces griseus ." Archives of Microbiology 151(4): 331-335. <http://hdl.handle.net/2027.42/46130> | en_US |
dc.identifier.issn | 0302-8933 | en_US |
dc.identifier.issn | 1432-072X | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/46130 | |
dc.description.abstract | An addition of cycloheximide to cycloheximide-producing Streptomyces griseus cultures resulted in reductions in the production rate and in the conversion of sugar into cycloheximide. In situ cycloheximide adsorption was observed to enhance: total cycloheximide titers; productivities; and the conversion of sugar to cycloheximide. During the secondary metabolite-producing phase, sugar consumption was observed to be linearly dependent on cycloheximide productivity. From this analysis a true product yield and maintenance coefficient were estimated to be 0.08 g cycloheximide/g glucose and 0.028 g glucose/g cell-h, respectively. The sixfold difference between this true product yield and a theoretical value obtained from knowledge of the biosynthetic pathway is discussed. Since the maintenance sugar requirement for cycloheximide production is large, stimulation of biosynthesis through in situ adsorption significantly increases the overall efficiency of sugar conversion to this secondary metabolite. | en_US |
dc.format.extent | 515517 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Springer-Verlag | en_US |
dc.subject.other | In Situ Adsorption | en_US |
dc.subject.other | Secondary Metabolism | en_US |
dc.subject.other | Ecology | en_US |
dc.subject.other | Microbiology | en_US |
dc.subject.other | Biochemistry, General | en_US |
dc.subject.other | Life Sciences | en_US |
dc.subject.other | Biotechnology | en_US |
dc.subject.other | Cell Biology | en_US |
dc.subject.other | Microbial Ecology | en_US |
dc.subject.other | In Situ Removal | en_US |
dc.subject.other | Cycloheximide | en_US |
dc.subject.other | Streptomyces Griseus | en_US |
dc.title | The effect of feedback regulation and in situ product removal on the conversion of sugar to cycloheximide by Streptomyces griseus | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Chemical Engineering, The University of Michigan, Dow Building, 48109, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationum | Department of Chemical Engineering, The University of Michigan, Dow Building, 48109, Ann Arbor, MI, USA; Department of Chemical Engineering and Center for Agricultural Biotechnology, University of Maryland Baltimore County, 21228, Baltimore, MD, USA | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/46130/1/203_2004_Article_BF00406560.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/BF00406560 | en_US |
dc.identifier.source | Archives of Microbiology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.