Identification of a sheath-associated protein involved in phosphate transport in Sphaerotilus natans
dc.contributor.author | Kulpa, Charles F. | en_US |
dc.contributor.author | Kaphammer, Bryan | en_US |
dc.date.accessioned | 2006-09-11T18:10:22Z | |
dc.date.available | 2006-09-11T18:10:22Z | |
dc.date.issued | 1990-11 | en_US |
dc.identifier.citation | Kaphammer, Bryan; Kulpa, Charles F.; (1990). "Identification of a sheath-associated protein involved in phosphate transport in Sphaerotilus natans ." Applied Microbiology and Biotechnology 34(2): 282-286. <http://hdl.handle.net/2027.42/46762> | en_US |
dc.identifier.issn | 1432-0614 | en_US |
dc.identifier.issn | 0175-7598 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/46762 | |
dc.description.abstract | Sphaerotilus natans was shown to have a fourfold lower K′ m of phosphate transport when grown in medium containing 0.1 m m phosphate, compared to cells grown in 10.0 m m phosphate. Analysis of sheath proteins from cells grown at these two phosphate levels revealed a protein of 53 kDa present in the sheath of cells grown at a phosphate concentration of 0.1 m m . This sheath-associated, phosphate-regulated protein, designated SapP, was gel purified and used to raise a polyclonal antibody. Enzyme-linked immunosorbent assay was used to localize this protein to the surface of the sheathed cells. Phosphate uptake assays done in the presence of the antibody also showed a rise in the K′ m of phosphate transport in cells grown in 0.1 m m phosphate, indicating that this protein is involved in high-affinity phosphate transport. | en_US |
dc.format.extent | 657782 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Springer-Verlag | en_US |
dc.subject.other | Biotechnology | en_US |
dc.subject.other | Microbial Genetics and Genomics | en_US |
dc.subject.other | Chemistry | en_US |
dc.subject.other | Microbiology | en_US |
dc.title | Identification of a sheath-associated protein involved in phosphate transport in Sphaerotilus natans | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Public Health | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biological Sciences, University of Notre Dame, 46556, Notre Dame, Indiana, USA; 1301 Catherine Medical Science II 5605, University of Michigan, 48109, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationother | Department of Biological Sciences, University of Notre Dame, 46556, Notre Dame, Indiana, USA | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/46762/1/253_2004_Article_BF00166797.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/BF00166797 | en_US |
dc.identifier.source | Applied Microbiology and Biotechnology | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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