High-dose, unlabeled, nonspecific antibody pretreatment: influence on specific antibody localization to human melanoma xenografts
dc.contributor.author | Liebert, Monica | en_US |
dc.contributor.author | Beierwaltes, William H. | en_US |
dc.contributor.author | Wilson, Barry S. | en_US |
dc.contributor.author | Wahl, Richard L. | en_US |
dc.date.accessioned | 2006-09-11T18:17:02Z | |
dc.date.available | 2006-09-11T18:17:02Z | |
dc.date.issued | 1987-06 | en_US |
dc.identifier.citation | Wahl, Richard L.; Wilson, Barry S.; Liebert, Monica; Beierwaltes, William H.; (1987). "High-dose, unlabeled, nonspecific antibody pretreatment: influence on specific antibody localization to human melanoma xenografts." Cancer Immunology Immunotherapy 24(3): 221-224. <http://hdl.handle.net/2027.42/46853> | en_US |
dc.identifier.issn | 1432-0851 | en_US |
dc.identifier.issn | 0340-7004 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/46853 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=3594484&dopt=citation | en_US |
dc.description.abstract | Nonspecific uptake of radiolabeled monoclonal antibodies in normal tissues is a significant problem for tumor imaging. A potential means of decreasing nonspecific antibody binding is to “blockade” nonspecific antibody binding sites by predosing with cold, nonspecific isotypematched antibody, before injecting specific antibody. Nontumor-specific murine monoclonal antibody LK2H10 (IgG1) or Ab-1 (IgG2a) was given i.v. at doses of 0 to 3.5 mg to nude mice with xenografts of human melanoma. These mice were then given i.v. 4 μg of 131 I anti-high molecular weight antigen of melanoma (HMWMAA) monoclonal antibody 763.24T (IgG1) or 225.28S (IgG2a), respectively. These mice were also given a tracer dose of 125 I LK2H10 or Ab-1, respectively. Specific tumor uptake of anti-HMWMAA antibodies was see in all cases. No drop in tumor or nontumor uptake was demonstrated for either of the tumor-specific or nonspecific monoclonal antibodies due to nonspecific monoclonal antibody pretreatment. These data suggest that high doses of isotype-matched unlabeled nonspecific monoclonal antibody given before 131 I tumor-specific monoclonal antibody, will not enhance tumor imaging. | en_US |
dc.format.extent | 429299 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Springer-Verlag | en_US |
dc.subject.other | Oncology | en_US |
dc.subject.other | Cancer Research | en_US |
dc.subject.other | Biomedicine | en_US |
dc.subject.other | Immunology | en_US |
dc.title | High-dose, unlabeled, nonspecific antibody pretreatment: influence on specific antibody localization to human melanoma xenografts | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Microbiology and Immunology | en_US |
dc.subject.hlbsecondlevel | Biological Chemistry | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Division of Nuclear Medicine, University of Michigan Medical Center, 1500 East Medical Center Drive, 48109-0028, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationum | Division of Nuclear Medicine, University of Michigan Medical Center, 1500 East Medical Center Drive, 48109-0028, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationum | Division of Nuclear Medicine, University of Michigan Medical Center, 1500 East Medical Center Drive, 48109-0028, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationother | Department of Pathology, Ann Arbor, Michigan, USA | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 3594484 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/46853/1/262_2004_Article_BF00205633.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/BF00205633 | en_US |
dc.identifier.source | Cancer Immunology Immunotherapy | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
Files in this item
Remediation of Harmful Language
The University of Michigan Library aims to describe library materials in a way that respects the people and communities who create, use, and are represented in our collections. Report harmful or offensive language in catalog records, finding aids, or elsewhere in our collections anonymously through our metadata feedback form. More information at Remediation of Harmful Language.
Accessibility
If you are unable to use this file in its current format, please select the Contact Us link and we can modify it to make it more accessible to you.