Chloroplast DNA from lettuce and Barnadesia (Asteraceae): structure, gene localization, and characterization of a large inversion
dc.contributor.author | Jansen, Robert K. | en_US |
dc.contributor.author | Palmer, Jeffrey D. | en_US |
dc.date.accessioned | 2006-09-11T18:25:42Z | |
dc.date.available | 2006-09-11T18:25:42Z | |
dc.date.issued | 1987-03 | en_US |
dc.identifier.citation | Jansen, Robert K; Palmer, Jeffrey D.; (1987). "Chloroplast DNA from lettuce and Barnadesia (Asteraceae): structure, gene localization, and characterization of a large inversion." Current Genetics 11 (6-7): 553-564. <http://hdl.handle.net/2027.42/46961> | en_US |
dc.identifier.issn | 0172-8083 | en_US |
dc.identifier.issn | 1432-0983 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/46961 | |
dc.description.abstract | We have cloned into plasmids 17 of 18 lettuce chloroplast DNA SacI fragments covering 96% of the genome. The cloned fragments were used to construct cleavage maps for 10 restriction enzymes for the chloroplast genomes of lettuce ( Lactuca sativa ) and Barnadesia caryophylla , two distantly related species in the sunflower family (Asteraceae). Both genomes are approximately 151 kb in size and contain a 25 kb inverted repeat. We also mapped the position and orientation of 37 chloroplast DNA genes. The mapping studies reveal that chloroplast DNAs of lettuce and Barnadesia differ by a 22 kb inversion in the large single copy region. Barnadesia has retained the primitive land plant genome arrangement, while the inversion has occurred in a lettuce lineage. The endpoints of the derived lettuce inversion were located by comparison to the well-characterized spinach and tobacco genomes. Both endpoints are located in intergenic spacers within tRNA gene clusters; one cluster being located downstream from the atpA gene and the other upstream from the psbD gene. The endpoint near the atpA gene is very close to one endpoint of a 20 kb inversion in wheat (Howe et al. 1983; Quigley and Weil 1985). Comparison of the restriction site maps gives an estimated sequence divergence of 3.7% for the lettuce and Barnadesia genomes. This value is relatively low compared to previous estimates for other angiosperm groups, suggesting a high degree of sequence conservation in the Asteraceae. | en_US |
dc.format.extent | 961775 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Springer-Verlag | en_US |
dc.subject.other | Plant Sciences | en_US |
dc.subject.other | Proteomics | en_US |
dc.subject.other | Inversion | en_US |
dc.subject.other | Gene Organization | en_US |
dc.subject.other | Microbiology | en_US |
dc.subject.other | Life Sciences | en_US |
dc.subject.other | Chloroplast DNA | en_US |
dc.subject.other | Biochemistry, General | en_US |
dc.subject.other | Cell Biology | en_US |
dc.subject.other | Microbial Genetics and Genomics | en_US |
dc.subject.other | Restriction Maps | en_US |
dc.subject.other | Asteraceae | en_US |
dc.title | Chloroplast DNA from lettuce and Barnadesia (Asteraceae): structure, gene localization, and characterization of a large inversion | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Natural Resources and Environment | en_US |
dc.subject.hlbsecondlevel | Molecular, Cellular and Developmental Biology | en_US |
dc.subject.hlbsecondlevel | Ecology and Evolutionary Biology | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.subject.hlbtoplevel | Science | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Department of Biology, University of Michigan, 48109, Ann Arbor, MI, USA; Department of Ecology and Evolutionary Biology, University of Connecticut, CT 06268, USA | en_US |
dc.contributor.affiliationum | Department of Biology, University of Michigan, 48109, Ann Arbor, MI, USA | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/46961/1/294_2004_Article_BF00384619.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/BF00384619 | en_US |
dc.identifier.source | Current Genetics | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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