Influence of the site of small bowel resection on intestinal epithelial cell apoptosis
dc.contributor.author | Yang, Hua | en_US |
dc.contributor.author | Haxhija, Emir Q. | en_US |
dc.contributor.author | Teitelbaum, Daniel H. | en_US |
dc.contributor.author | Spencer, Ariel U. | en_US |
dc.contributor.author | Sun, Xiaoyi | en_US |
dc.date.accessioned | 2006-09-11T18:40:38Z | |
dc.date.available | 2006-09-11T18:40:38Z | |
dc.date.issued | 2006-01 | en_US |
dc.identifier.citation | Haxhija, Emir Q.; Yang, Hua; Spencer, Ariel U.; Sun, Xiaoyi; Teitelbaum, Daniel H.; (2006). "Influence of the site of small bowel resection on intestinal epithelial cell apoptosis." Pediatric Surgery International 22(1): 37-42. <http://hdl.handle.net/2027.42/47176> | en_US |
dc.identifier.issn | 1437-9813 | en_US |
dc.identifier.issn | 0179-0358 | en_US |
dc.identifier.uri | https://hdl.handle.net/2027.42/47176 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=16307277&dopt=citation | en_US |
dc.description.abstract | Massive small bowel resection (SBR) results in a significant increase in intestinal epithelial cell (EC) proliferation as well as apoptosis. Because the site of SBR (proximal (P) vs. distal (D)) affects the degree of intestinal adaptation, we hypothesized that different rates of EC apoptosis would also be found between P-SBR and D-SBR models. Wild-type C57BL/6J mice underwent: (1) 60% P-SBR, (2) 60% D-SBR, or (3) SHAM-operation (transaction–reanastomosis) at the mid-gut point. Mice were sacrificed after 7 days. EC apoptosis was measured by TUNEL staining. EC-related apoptotic gene expression including intrinsic and extrinsic pathways was measured with reverse transcriptase-polymerase chain reaction. Bcl-2 and bax protein expression were analyzed by Western immunobloting. Both models of SBR led to significant increases in villus height and crypt depth; however, the morphologic adaptation was significantly higher after P-SBR compared to D-SBR ( P <0.01). Both models of SBR led to significant increases in enterocyte apoptotic rates compared to respective sham levels; however, apoptotic rates were 2.5-fold higher in ileal compared to jejunal segments ( P <0.01). P-SBR led to significant increases in bax (pro-apoptotic) and Fas expression, whereas D-SBR resulted in a significant increase in TNF-α expression ( P <0.01). EC apoptosis seems to be an important component of intestinal adaptation. The significant difference in EC apoptotic rates between proximal and distal intestinal segments appeared to be due to utilization of different mechanisms of action. | en_US |
dc.format.extent | 282686 bytes | |
dc.format.extent | 3115 bytes | |
dc.format.mimetype | application/pdf | |
dc.format.mimetype | text/plain | |
dc.language.iso | en_US | |
dc.publisher | Springer-Verlag | en_US |
dc.subject.other | Apoptosis | en_US |
dc.subject.other | Short Bowel Syndrome | en_US |
dc.subject.other | Small Bowel Resection | en_US |
dc.subject.other | Bcl-2 | en_US |
dc.subject.other | Bax | en_US |
dc.subject.other | Intestine | en_US |
dc.title | Influence of the site of small bowel resection on intestinal epithelial cell apoptosis | en_US |
dc.type | Article | en_US |
dc.subject.hlbsecondlevel | Pediatrics | en_US |
dc.subject.hlbtoplevel | Health Sciences | en_US |
dc.description.peerreviewed | Peer Reviewed | en_US |
dc.contributor.affiliationum | Section of Pediatric Surgery, Department of Surgery, University of Michigan Medical School, and C. S. Mott Children’s Hospital, Mott F 3970, P.O. Box 0245, Ann Arbor, MI, 48109 , USA, | en_US |
dc.contributor.affiliationum | Section of Pediatric Surgery, Department of Surgery, University of Michigan Medical School, and C. S. Mott Children’s Hospital, Mott F 3970, P.O. Box 0245, Ann Arbor, MI, 48109 , USA, | en_US |
dc.contributor.affiliationum | Section of Pediatric Surgery, Department of Surgery, University of Michigan Medical School, and C. S. Mott Children’s Hospital, Mott F 3970, P.O. Box 0245, Ann Arbor, MI, 48109 , USA, | en_US |
dc.contributor.affiliationum | Section of Pediatric Surgery, Department of Surgery, University of Michigan Medical School, and C. S. Mott Children’s Hospital, Mott F 3970, P.O. Box 0245, Ann Arbor, MI, 48109 , USA, | en_US |
dc.contributor.affiliationother | Department of Pediatric Surgery, Medical University Graz, Graz, Austria, | en_US |
dc.contributor.affiliationumcampus | Ann Arbor | en_US |
dc.identifier.pmid | 16307277 | en_US |
dc.description.bitstreamurl | http://deepblue.lib.umich.edu/bitstream/2027.42/47176/1/383_2005_Article_1576.pdf | en_US |
dc.identifier.doi | http://dx.doi.org/10.1007/s00383-005-1576-5 | en_US |
dc.identifier.source | Pediatric Surgery International | en_US |
dc.owningcollname | Interdisciplinary and Peer-Reviewed |
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